André Weibson Paz Pinheiro, Cavalcante Géssica Soares, Ribeiro Wesley Lyeverton Correia, Santos Jessica Maria Leite Dos, Macedo Iara Tersia Freitas, Paula Haroldo César Beserra de, Morais Selene Maia de, Melo Janaina Viana de, Bevilaqua Claudia Maria Leal
Programa de Pós-graduação em Ciências Veterinárias, Faculdade de Veterinária - FAVET, Universidade Estadual do Ceará - UECE, Fortaleza, CE, Brasil.
Departamento de Química Analítica e Físico-Química, Universidade Federal do Ceará - UFC, Fortaleza, CE, Brasil.
Rev Bras Parasitol Vet. 2017 Jul-Sep;26(3):323-330. doi: 10.1590/S1984-29612017056.
Thymol is a monoterpene and acetylation form of this compound can reduce the toxicity and enhance its biological effects. The objective of this study was to evaluate the effect of thymol and thymol acetate (TA) on egg, larva and adult Haemonchus contortus and the cuticular changes, acute toxicity in mice and the efficacy on sheep gastrointestinal nematodes. In vitro tests results were analyzed by analysis of variance (ANOVA) and followed by comparison with Tukey test or Bonferroni. The efficacy of in vivo test was calculated by the BootStreet program. In the egg hatch test (EHT), thymol (0.5 mg/mL) and TA (4 mg/mL) inhibited larval hatching by 98% and 67.1%, respectively. Thymol and TA (8 mg/mL) inhibited 100% of larval development. Thymol and TA (800 µg/mL) reduced the motility of adult worms, by 100% and 83.4%, respectively. Thymol caused cuticular changes in adult worm teguments. In the acute toxicity test, the LD50 of thymol and TA were 1,350.9 mg/kg and 4,144.4 mg/kg, respectively. Thymol and TA reduced sheep egg count per gram of faeces (epg) by 59.8% and 76.2%, respectively. In in vitro tests thymol presented better anthelmintic activity than TA. However TA was less toxic and in in vivo test efficacy was similar.
百里香酚是一种单萜类化合物，该化合物的乙酰化形式可降低其毒性并增强其生物学效应。本研究的目的是评估百里香酚和醋酸百里香酚（TA）对捻转血矛线虫的虫卵、幼虫和成虫的影响以及表皮变化、对小鼠的急性毒性和对绵羊胃肠道线虫的疗效。体外试验结果采用方差分析（ANOVA）进行分析，随后与Tukey检验或Bonferroni检验进行比较。体内试验的疗效通过BootStreet程序计算。在虫卵孵化试验（EHT）中，百里香酚（0.5 mg/mL）和TA（4 mg/mL）分别抑制幼虫孵化98%和67.1%。百里香酚和TA（8 mg/mL）抑制幼虫发育达100%。百里香酚和TA（800 µg/mL）分别使成虫活动能力降低100%和83.4%。百里香酚导致成虫体表出现表皮变化。在急性毒性试验中，百里香酚和TA的半数致死量（LD50）分别为1350.9 mg/kg和4144.4 mg/kg。百里香酚和TA分别使绵羊每克粪便中的虫卵数（epg）减少59.8%和76.2%。在体外试验中，百里香酚的驱虫活性优于TA。然而，TA的毒性较小，且在体内试验中的疗效相似。
