Antonio Evelyn Louise, Nascimento Aguinaldo José do, Lima Antonio Adilson Soares de, Leonart Maria Suely Soares, Fernandes Ângela
Prefeitura Municipal de Curitiba, Curitiba, PR, Brasil.
Universidade Federal do Paraná (UFPR), Curitiba, PR, Brasil.
Rev Paul Pediatr. 2017 Jul-Sep;35(3):296-301. doi: 10.1590/1984-0462/;2017;35;3;00010. Epub 2017 Jul 20.
To assess the genotoxicity and cytotoxicity produced by X-rays in the epithelium of the oral mucosa of infants exposed to panoramic radiography.
The sample consisted of 30 healthy children, 19 females and 11 males, ranging in age from 4 to 10 years (average of 7 years of age). Oral mucosa cells were collected by liquid-based cytology immediately before and after seven days following the exposure to panoramic radiography. Smears were processed and stained using the modified Feulgen Rossenbeck technique. Bud and broken egg nuclear projections, changes in the form of micronuclei, and genotoxic and cytotoxic changes of pyknosis, karyorrhexis and karyolysis were analyzed and quantified.
The frequency of pyknosis, buds and broken eggs was significantly higher after exposure to X-rays (p<0.05), but there was no statistically significant difference regarding gender, as well as in the other changes studied.
Exposure to X-rays emitted during submission to panoramic radiography may induce cell death in the epithelium of children's oral mucosa. No evidence was found for a significant genotoxic effect.
评估全景X线摄影对婴儿口腔黏膜上皮产生的遗传毒性和细胞毒性。
样本包括30名健康儿童，19名女性和11名男性，年龄在4至10岁之间（平均7岁）。在全景X线摄影前后7天，通过液基细胞学方法收集口腔黏膜细胞。涂片采用改良的福尔根-罗森贝克技术进行处理和染色。分析并量化芽状和破碎卵核突起、微核形式的变化以及核固缩、核碎裂和核溶解的遗传毒性和细胞毒性变化。
X线照射后核固缩、芽状和破碎卵的频率显著更高（p<0.05），但在性别以及所研究的其他变化方面无统计学显著差异。
接受全景X线摄影时所受的X线照射可能会诱导儿童口腔黏膜上皮细胞死亡。未发现明显遗传毒性作用的证据。
