Saha B C, Kennedy G J
Bioenergy Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, Peoria, IL, USA.
Lett Appl Microbiol. 2017 Dec;65(6):527-533. doi: 10.1111/lam.12810. Epub 2017 Oct 29.
Itaconic acid (IA), an unsaturated 5-carbon dicarboxylic acid, is a building block platform chemical that is currently produced industrially from glucose by fermentation with Aspergillus terreus. Softwood has the potential to serve as low cost source of sugars for its production. Effective utilization of all softwood derived sugars such as glucose, mannose and galactose by the fungus for production of IA will lower the cost of its production. In this work, 20 A. terreus strains were evaluated for the first time for IA production from mannose and galactose in shake-flasks at initial pH of 3·1, 33°C and 200 rev min for 7 days. Strain NRRL 1971 possesses the unique ability to produce high concentrations of IA from mannose. It produced 36·4 ± 0·2 g IA from 80 g mannose per litre with a yield of 0·46 g g mannose (highest titre reported so far). This strain has the potential to be used for IA production from softwood. The maximum (1·1 ± 0·2 g) IA was produced by strain DSM 23081 from 80 g galactose per litre utilizing only 9·1 ± 0·3 g. Galactose was not suitable for IA production by these strains. This is the first detailed report on the production of IA from mannose and galactose.
Itaconic acid (IA) is a building block platform chemical which is currently produced industrially from glucose by fermentation with Aspergillus terreus. In order to expand the use of IA, its production cost must be lowered. Softwood has the potential to serve as low cost source of sugars for its production. In this work, 20 A. terreus strains were evaluated for the first time for production of IA from mannose and galactose, sugars derived from softwood. A novel strain was found that gave the highest IA titre reported so far. Galactose was a poor substrate for IA production by A. terreus.
衣康酸（IA）是一种不饱和的5碳二羧酸，是一种基础平台化学品，目前通过土曲霉发酵由葡萄糖工业生产。软木有潜力作为其生产的低成本糖源。真菌有效利用所有源自软木的糖（如葡萄糖、甘露糖和半乳糖）来生产IA将降低其生产成本。在这项工作中，首次在摇瓶中于初始pH值为3.1、33°C和200转/分钟的条件下，对20株土曲霉菌株利用甘露糖和半乳糖生产IA进行了7天的评估。菌株NRRL 1971具有从甘露糖生产高浓度IA的独特能力。它从每升80克甘露糖中生产出36.4±0.2克IA，产率为0.46克/克甘露糖（是迄今为止报道的最高产量）。该菌株有潜力用于从软木生产IA。菌株DSM 23081从每升80克半乳糖中仅利用9.1±0.3克生产出最大量（1.1±0.2克）IA。半乳糖不适合这些菌株用于IA生产。这是关于从甘露糖和半乳糖生产IA的第一份详细报告。
衣康酸（IA）是一种基础平台化学品，目前通过土曲霉发酵由葡萄糖工业生产。为了扩大IA的用途，必须降低其生产成本。软木有潜力作为其生产的低成本糖源。在这项工作中，首次对20株土曲霉菌株利用软木衍生的糖甘露糖和半乳糖生产IA进行了评估。发现了一种新菌株，其IA产量是迄今为止报道的最高值。半乳糖是土曲霉生产IA的不良底物。
