Saha Badal C, Kennedy Gregory J
U. S. Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research, Bioenergy Research Unit, Peoria, IL 61604, USA.
U. S. Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research, Bioenergy Research Unit, Peoria, IL 61604, USA.
J Microbiol Methods. 2018 Jan;144:53-59. doi: 10.1016/j.mimet.2017.11.002. Epub 2017 Nov 3.
Itaconic acid (IA) is a building block platform chemical that is currently produced industrially from glucose by fermentation with Aspergillus terreus. However, lignocellulosic biomass has the potential to serve as low cost source of sugars for production of IA. Previously, 100 A. terreus strains were evaluated for production of IA from pentose sugars in shake-flasks. Six selected strains were then investigated for IA production in shake-flasks. But none of the strains grew and produced IA using biomass hydrolyzates. In order to study the factors inhibiting fungal growth and IA production, we have evaluated these six strains for sugar utilization and IA production from glucose, xylose, arabinose, mixed sugars, and both dilute acid and liquid hot water pretreated wheat straw hydrolyzates in microtiter plate (MTP) microbioreactors at 100μL scale. The results clearly indicate that MTP is very useful as a convenient, reliable and affordable platform to investigate the reasons for inhibition of growth and IA production by the A. terreus strains and should greatly aid in strain development and optimization of IA production by the fungal strains.
衣康酸（IA）是一种基础平台化学品，目前在工业上通过用土曲霉发酵葡萄糖来生产。然而，木质纤维素生物质有潜力作为生产衣康酸的低成本糖源。此前，对100株土曲霉菌株进行了摇瓶中由戊糖生产衣康酸的评估。然后对6株筛选出的菌株进行了摇瓶中衣康酸生产的研究。但没有一个菌株能利用生物质水解产物生长并生产衣康酸。为了研究抑制真菌生长和衣康酸生产的因素，我们在100μL规模的微量滴定板（MTP）微生物反应器中，对这6株菌株利用葡萄糖、木糖、阿拉伯糖、混合糖以及稀酸和液态热水预处理的小麦秸秆水解产物进行糖利用和衣康酸生产的评估。结果清楚地表明，MTP作为一个方便、可靠且经济实惠的平台，对于研究土曲霉菌株生长抑制和衣康酸生产抑制的原因非常有用，并且应该极大地有助于菌株开发以及真菌菌株衣康酸生产的优化。
