Butchaiah G
Department of Veterinary Virology and Immunology, Royal Veterinary and Agricultural University, Copenhagen V, Denmark.
Acta Virol. 1988 Jan;32(1):60-4.
Three different methods, namely plaque assay, immunofluorescent cell (IFC) count and end-point dilution (TCID50) were evaluated for quantitative infectivity assay of the cell culture adapted UK strain of bovine rotavirus in secondary calf kidney (CK) cells and BGM cell line. Plaque and IFC count techniques were found equally efficient for infectivity titration of bovine rotavirus. Addition of trypsin into maintenance medium enhanced the sensitivity of the TCID50 method. Both CK and BGM cells served as efficient assay cells for infectivity assay of bovine rotavirus by IFC count and TCID50 methods, whereas, for plaque assay, only CK cells were found suitable.
为了对适应细胞培养的英国牛轮状病毒株在二代犊牛肾（CK）细胞和BGM细胞系中进行定量感染性测定，评估了三种不同的方法，即蚀斑测定法、免疫荧光细胞（IFC）计数法和终点稀释法（TCID50）。发现蚀斑和IFC计数技术在牛轮状病毒感染性滴定方面同样有效。在维持培养基中添加胰蛋白酶可提高TCID50方法的灵敏度。通过IFC计数法和TCID50方法，CK细胞和BGM细胞均作为牛轮状病毒感染性测定的有效测定细胞，而对于蚀斑测定法，仅发现CK细胞适用。
