Schulze P, Schumacher B
Acta Histochem Suppl. 1986;33:289-92.
Regardless of different host cells and trypsin addition to inoculum, three rotavirus strains (human rotavirus strain Wa, SA 11 virus, bovine rotavirus) displayed the same features in adsorption, endocytosis at 30 min p.i. and the following steps of morphogenesis. The first progeny virus was regularly observed in the RER cisternae at 5 h p.i. It appears that virus yield enhancement by addition of trypsin was due to a greater number of cells having been infected. The infected single cell did not produce more virus particles. Crystalloid arrangement of provirus particles in viroplasm was only observed with the strain Wa virus. The strain Wa and bovine rotavirus produced intracytoplasmic and intranuclear tubes. SA 11 virus induced only intranuclear striated ribbons.
无论宿主细胞有何不同，也无论接种物中是否添加胰蛋白酶，三种轮状病毒株（人轮状病毒Wa株、SA 11病毒、牛轮状病毒）在吸附、感染后30分钟的内吞作用以及随后的形态发生步骤中均表现出相同特征。感染后5小时，首次在粗面内质网池内定期观察到子代病毒。添加胰蛋白酶提高病毒产量似乎是由于更多细胞受到感染。被感染的单个细胞产生的病毒颗粒并未增多。仅在Wa株病毒中观察到病毒前体颗粒在病毒质内呈晶体状排列。Wa株和牛轮状病毒产生胞质内和核内管。SA 11病毒仅诱导核内条纹状带。
