Myers T J, Schat K A
Department of Avian and Aquatic Animal Medicine, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853.
Avian Dis. 1989 Jul-Sep;33(3):578-81.
The Ch2 strain of avian rotavirus was propagated in primary chick kidney cell (CKC) and MA104 cell cultures in the presence of trypsin. Cultures were evaluated for the presence of rotavirus by an indirect fluorescent antibody (IFA) assay and by an indirect enzyme-linked immunosorbent assay (ELISA). After two passages, the viral titer was significantly higher in CKC than MA104 cell cultures. Also, the IFA assay was more sensitive than the indirect ELISA for detecting rotavirus-positive cultures.
禽轮状病毒Ch2株在胰蛋白酶存在的情况下，在原代鸡肾细胞（CKC）和MA104细胞培养物中增殖。通过间接荧光抗体（IFA）试验和间接酶联免疫吸附试验（ELISA）评估培养物中是否存在轮状病毒。传代两次后，CKC中的病毒滴度显著高于MA104细胞培养物。此外，IFA试验在检测轮状病毒阳性培养物方面比间接ELISA更敏感。
