College of Veterinary Medicine and Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, China.
Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China.
Transbound Emerg Dis. 2018 Apr;65(2):432-440. doi: 10.1111/tbed.12721. Epub 2017 Sep 30.
Porcine postweaning multisystemic wasting syndrome (PMWS) caused by porcine circovirus type 2 (PCV2) is a disease causing severe economic losses annually worldwide to the pig industry. PCV2 infection was first reported in China in 2000, and currently has three major genotypes, PCV2a, b and d, circulating in this country. To further elucidate the origin and prevalence of PCV2 in China, 123 clinical pig tissue samples collected in 25 provinces between 1990 and 1999 were analysed by PCV2-specific PCR, resulting in identification of 23 PCV2 strains collected between 1996 and 1999. Phylogenetic analysis based on the nucleotide sequences of open reading frame 2 (ORF2) showed that 20 of the 23 grouped within PCV2a, while the remaining three strains formed an independent clade, so far unreported and therefore named PCV2f. This genotype shared lower sequence identity with other known genotypes. This study provides further understanding of the genetic diversity and evolution of PCV2 and has tracked PCV2 infection in China back to 1996 rather than 2000.
猪断奶后多系统消耗综合征（PMWS）由猪圆环病毒 2 型（PCV2）引起，是一种每年给全球养猪业造成严重经济损失的疾病。PCV2 感染于 2000 年在中国首次报道，目前在中国流行的有三个主要基因型，即 PCV2a、b 和 d。为了进一步阐明 PCV2 在我国的起源和流行情况，对 1990 年至 1999 年间在 25 个省采集的 123 份临床猪组织样本进行了 PCV2 特异性 PCR 分析，鉴定出 1996 年至 1999 年间采集的 23 株 PCV2 毒株。基于开放阅读框 2（ORF2）核苷酸序列的系统进化分析表明，23 株中有 20 株属于 PCV2a，其余 3 株形成一个独立的分支，迄今尚未报道，因此命名为 PCV2f。该基因型与其他已知基因型的序列同一性较低。本研究进一步了解了 PCV2 的遗传多样性和进化，并将 PCV2 在我国的感染追溯到 1996 年，而不是 2000 年。
