Maham Mehdi, Karami-Osboo Rouhollah
Department of Chemistry, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul, Iran.
Mycotoxins Research Laboratory, Iranian Research Institute of Plant Protection, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran.
Iran J Pharm Res. 2017 Spring;16(2):462-470.
The application of Pd/FeO nanoparticles (NPs) for the adsorption of sulfathiazole (STZ) from urine samples prior to high performance liquid chromatography-ultraviolet detection was studied. Pd/FeO NPs were synthesized using plant extract. Possible impact parameters in the extraction process such as magnetic adsorbents amount, extraction time, sample pH, and desorption conditions were investigated and optimized. Under the optimum conditions, the detection and quantification limits were 10 and 30 ng mL, respectively. The relative standard deviation for five measurements of 100 ng mL of STZ was 5.8 %. The proposed method was used for the analysis of different urine samples, and acceptable recoveries in the range of 87.6 - 101.3% were obtained. These results indicated that biosynthesized Pd/FeO NPs can be used as an efficient adsorbent for extraction of sulfathiazole from urine samples.
研究了钯/氧化亚铁纳米颗粒（NPs）在高效液相色谱-紫外检测之前用于从尿液样本中吸附磺胺噻唑（STZ）的应用。钯/氧化亚铁纳米颗粒采用植物提取物合成。研究并优化了萃取过程中可能的影响参数，如磁性吸附剂用量、萃取时间、样品pH值和解吸条件。在最佳条件下，检测限和定量限分别为10和30 ng/mL。对100 ng/mL的磺胺噻唑进行五次测量的相对标准偏差为5.8%。所提出的方法用于分析不同的尿液样本，回收率在87.6 - 101.3%范围内，结果令人满意。这些结果表明，生物合成的钯/氧化亚铁纳米颗粒可作为从尿液样本中萃取磺胺噻唑的有效吸附剂。
