Fallah Esmaeil, Jafarpour Zahra, Mahami-Oskouei Mahmoud, Haghighi Ali, Niyyati Maryam, Spotin Adel, Khezri Aram
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Dept. of Parasitology and Mycology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Iran J Parasitol. 2017 Jul-Sep;12(3):355-363.
is an opportunistic amphizoic protozoan found in different fresh water sources. The aim of this study was to identify and characterize isolates from surface resting waters, in Northwest Iran.
Samples were collected from twenty-two different areas, between May and Sep 2014. After filtration, samples were cultivated on non-nutrient agar. The extracted DNAs were amplified and sequenced using partial 18S rRNA in order to genotype and phylogenetic analyses.
Thirty-four (68%) out of 50 collected samples were positive for free-living amoebae based on both culture and morphological characterizations but 28 samples were identified as spp. by PCR. Sequentially, one isolate was identified as , (T5) (AN: KP940443, identity 99.7%-100%, and divergence 0.3%) whilst other sequenced isolates identified spp. (AN: KP940444-45) as very similar to and with identity 100% and divergence 0%.
Surface resting waters in Northwest Iran, were potentially contaminated with pathogenic amphizoic protozoan. Further studies will be required to determine other species and genotypes in the region.
是一种在不同淡水水源中发现的机会性兼性原生动物。本研究的目的是鉴定和表征伊朗西北部地表静水中的分离株。
于2014年5月至9月间从22个不同地区采集样本。过滤后，将样本接种于无营养琼脂上培养。提取的DNA使用部分18S rRNA进行扩增和测序，以进行基因分型和系统发育分析。
根据培养和形态学特征，50份采集样本中有34份（68%）自由生活阿米巴呈阳性，但28份样本经PCR鉴定为 spp.。随后，一株分离株被鉴定为 ，（T5）（登录号：KP940443，同一性99.7%-100%，差异0.3%），而其他测序分离株鉴定为 spp.（登录号：KP940444-45），与 和 非常相似，同一性为100%，差异为0%。
伊朗西北部的地表静水中可能被致病性兼性原生动物污染。需要进一步研究以确定该地区其他的物种和基因型。
