Khezri Aram, Fallah Esmaeel, Mostafazadeh Mostafa, Spotin Adel, Shahbazi Abbas, Mahami-Oskouei Mahmoud, Hazratian Taimuor
Department of Parasitology and Mycology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran; Students' Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Parasitology and Mycology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran; Immunology Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Laboratory Medicine, Ardabil Medical Sciences Branch, Islamic Azad University, Ardabil, Iran.
Jundishapur J Microbiol. 2016 Oct 18;9(11):e38481. doi: 10.5812/jjm.38481. eCollection 2016 Nov.
spp. is a free-living opportunistic protozoan parasites, which can be found in tap, fresh and bottled mineral waters, contact lens solutions, soil etc.
The present study is aimed to determine the spp. on the basis of their morpho-molecular aspects in different water sources of the West Azerbaijan province, Northwest of Iran.
In this cross-sectional study, 60 water samples were collected from rivers and tap waters during June to September 2015. The water samples were filtered through a cellulose nitrate filter and cultured on non-nutrient agar medium. The extracted DNAs were amplified and some ampliqons were sequenced using partial 18S rRNA for genotyping and phylogenetic analyses.
Twenty-seven (45%) out of 60 water samples were positive to spp. using both culture and morphological examinations. In addition, 24 (40%) out of 27 positive samples in culture method were confirmed by PCR to be spp.
A relatively high prevalence of spp. in rivers reflects a risk alert for threatening human health in the region. However, well hygienic status of the tap waters considering spp. cannot be ignored in western co-border regions of Iran-Iraq. This study can also serve as a platform for further explorations of water sources in Iran and neighboring countries.
某属（spp.）是一种自由生活的机会性原生动物寄生虫，可在自来水、淡水和瓶装矿泉水中、隐形眼镜护理液、土壤等中发现。
本研究旨在根据伊朗西北部西阿塞拜疆省不同水源中某属的形态分子特征来确定该属。
在这项横断面研究中，于2015年6月至9月从河流和自来水中采集了60份水样。水样通过硝酸纤维素滤膜过滤，并在无营养琼脂培养基上培养。提取的DNA进行扩增，部分扩增子使用部分18S rRNA进行测序以进行基因分型和系统发育分析。
60份水样中有27份（45%）通过培养和形态学检查对某属呈阳性。此外，培养法检测出的27份阳性样本中有24份（40%）经PCR确认为某属。
河流中某属的相对高流行率反映了该地区对人类健康构成威胁的风险警报。然而，在伊朗 - 伊拉克西部边境地区，考虑到某属，自来水良好的卫生状况也不容忽视。本研究也可为进一步探索伊朗及周边国家的水源提供一个平台。
