Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Water Health. 2011 Sep;9(3):603-8. doi: 10.2166/wh.2011.152.
A comprehensive survey assessing the presence of Acanthamoeba was conducted on 50 samples from water sources in parks and public squares from 22 municipal districts of Tehran, Iran. The prevalence and genotypes of Acanthamoeba were determined by PCR and the PCR fragments of ribosomal RNA genes sequenced. Sixteen (32%) samples were positive for Acanthamoeba spp. Sequence analysis revealed that the positive isolates belonged to the T4 and T5 genotypes. Fourteen isolates (87.5%) were T4, and two (12.5%) were T5. Acanthamoeba may be a problematic organism for contact lens wearers and for immunocompromised individuals. In Iran, Acanthamoeba keratitis has increased in recent years, mainly due to poor hygiene in contact lens wearers. A thorough survey for the prevalence of this amoeba could have a significant role in prevention of disease. This is the first report of the T5 genotype from water in recreational areas of Tehran.
对伊朗德黑兰 22 个行政区的公园和公共广场的 50 个水源样本进行了一项全面调查，以评估棘阿米巴的存在情况。通过 PCR 确定棘阿米巴的流行率和基因型，并对核糖体 RNA 基因的 PCR 片段进行测序。16 个（32%）样本对棘阿米巴属呈阳性。序列分析显示，阳性分离株属于 T4 和 T5 基因型。14 个分离株（87.5%）为 T4，2 个（12.5%）为 T5。棘阿米巴可能是隐形眼镜佩戴者和免疫功能低下者的问题生物体。在伊朗，棘阿米巴角膜炎近年来有所增加，主要是由于隐形眼镜佩戴者卫生条件差。对这种阿米巴的流行情况进行全面调查可能对疾病预防具有重要意义。这是德黑兰娱乐区水中 T5 基因型的首次报道。
