Comparison of Composition and Anticaries Effect of Extracts with Different Isolation Methods.

OBJECTIVES

water extract (GCE) has been demonstrated to inhibit dental caries by favorably shifting the demineralization/remineralization balance of enamel and inhibiting the biomass and acid formation of dental biofilm. The present study focused on the comparison of composition and anticaries effect of extracts with different isolation methods, aiming to improve the efficacy of caries prevention.

METHODS

The composition of water extract (GCE), ethanol extract (eGCE) and commercial tannic acid was compared. High performance liquid chromatography coupled to electrospray ionization-time of flight-mass spectrometry (HPLC-ESI-TOF-MS) analysis was used to analyze the main ingredients. pH-cycling regime and polymicrobial biofilms model were used to assess the ability of different extracts to inhibit enamel demineralization, acid formation and biofilm formation.

RESULTS

All the GCE, eGCE and tannic acid contained a high level of total phenolics. HPLC-ESI-TOF-MS analysis showed that the main ingredients of GCE were gallic acid (GA), while eGCE mainly contained 4-7 galloylglucopyranoses (GGs) and tannic acid mainly contained 5-10 GGs. Furthermore, eGCE and tannic acid showed a better effect on inhibiting enamel demineralization, acid formation and biofilm formation compared to GCE.

CONCLUSIONS

extracts with higher tannin content were suggested to have higher potential to prevent dental caries.