Francipane Maria Giovanna, Lagasse Eric
McGowan Institute for Regenerative Medicine, Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, USA.
Ri.MED Foundation, 90133 Palermo, Italy.
Curr Pathobiol Rep. 2016 Sep;4(3):77-85. doi: 10.1007/s40139-016-0111-9. Epub 2016 Jul 2.
In recent years, functional three-dimensional (3D) tissue generation in vitro has been significantly advanced by tissue-engineering methods, achieving better reproduction of complex native organs compared to conventional culture systems. This review will discuss traditional 3D cell culture techniques as well as newly developed technology platforms. These recent techniques provide new possibilities in the creation of human body parts and provide more accurate predictions of tissue response to drug and chemical challenges. Given the rapid advancement in the human induced pluripotent stem cell (iPSC) field, these platforms also hold great promise in the development of patient-specific, transplantable tissues and organs on demand.
近年来，通过组织工程方法，体外功能性三维（3D）组织生成取得了显著进展，与传统培养系统相比，能更好地再现复杂的天然器官。本综述将讨论传统的3D细胞培养技术以及新开发的技术平台。这些最新技术为制造人体部件提供了新的可能性，并能更准确地预测组织对药物和化学刺激的反应。鉴于人类诱导多能干细胞（iPSC）领域的快速发展，这些平台在按需开发患者特异性、可移植组织和器官方面也具有巨大潜力。
