Iosub Gheorghe, Lungescu Ioana-Alexandra, Bîrcă Alexandra Cătălina, Niculescu Adelina-Gabriela, Balaure Paul Catalin, Constantinescu Sorin, Mihaiescu Bogdan, Rădulescu Dragoș Mihai, Grumezescu Alexandru Mihai, Hudiță Ariana, Neacșu Ionela Andreea, Rădulescu Adrian Radu
Faculty of Medicine, Carol Davila University of Medicine and Pharmacy, 8 Eroii Sanitari Street, 050474 Bucharest, Romania.
Department of Science and Engineering of Oxide Materials and Nanomaterials, University Politehnica of Bucharest, 1-7 Gh. Polizu Street, 060042 Bucharest, Romania.
Materials (Basel). 2025 Mar 8;18(6):1206. doi: 10.3390/ma18061206.
Osteoporosis and bone defects are commonly observed in postmenopausal women, often linked to decreased folic acid levels, which play a crucial role in bone metabolism and regeneration. This study investigates 3D-printed polyethylene terephthalate glycol (PETG)-based porous scaffolds impregnated with chitosan (CS), hydroxyapatite (HAp), and folic acid (FA) for bone tissue engineering applications. The PETG-CS scaffold serves as the primary structural framework, with HAp incorporated to enhance bioactivity through its osteoconductive and osteoinductive properties. FA was included to address potential deficiencies in bone quality and to stimulate cellular differentiation. The scaffolds were fabricated using precise 3D printing techniques, yielding structures with controlled porosity. Physicochemical analyses confirmed the successful integration of HAp and FA into the PETG-CS matrix. Biological evaluations using preosteoblast cell lines demonstrated enhanced cell viability, proliferation, and biocompatibility of the scaffolds. These findings highlight the promising applications of PETG-CS-HAp-FA scaffolds in bone tissue engineering, providing a platform for future investigations into personalized regenerative therapies.
骨质疏松症和骨缺损在绝经后女性中很常见，通常与叶酸水平降低有关，而叶酸在骨代谢和再生中起着至关重要的作用。本研究调查了用于骨组织工程应用的3D打印的基于聚对苯二甲酸乙二醇酯二醇（PETG）的多孔支架，该支架浸渍了壳聚糖（CS）、羟基磷灰石（HAp）和叶酸（FA）。PETG-CS支架作为主要的结构框架，加入HAp以通过其骨传导性和骨诱导性增强生物活性。加入FA是为了解决骨质量方面的潜在缺陷并刺激细胞分化。这些支架采用精确的3D打印技术制造，产生具有可控孔隙率的结构。物理化学分析证实HAp和FA成功整合到PETG-CS基质中。使用前成骨细胞系进行的生物学评估表明，支架的细胞活力、增殖和生物相容性增强。这些发现突出了PETG-CS-HAp-FA支架在骨组织工程中的应用前景，为未来个性化再生疗法的研究提供了一个平台。
