Lench N, Stanier P, Williamson R
Department of Biochemistry and Molecular Genetics, St Mary's Hospital Medical School, London.
Lancet. 1988 Jun 18;1(8599):1356-8. doi: 10.1016/s0140-6736(88)92178-2.
A technique is described which demonstrates that sufficient human DNA for direct gene analysis can be isolated from buccal epithelial cells obtained by mouthwash. This procedure is much simpler and cheaper than existing methods and, combined with DNA amplification by polymerase chain reaction, should allow community screening for carrier status for single gene defects such as cystic fibrosis.
本文描述了一种技术，该技术表明可以从通过漱口获得的颊上皮细胞中分离出足够用于直接基因分析的人类DNA。该程序比现有方法更简单、更便宜，并且与通过聚合酶链反应进行的DNA扩增相结合，应该可以对诸如囊性纤维化等单基因缺陷的携带者状态进行社区筛查。
