与感染I型人类T细胞白血病病毒个体血清发生免疫反应的大肠杆菌中蛋白质的合成

Synthesis of proteins in Escherichia coli immunoreactive with sera from individuals infected with human T-cell leukemia virus type I.

作者信息

Kitajima T, Itamura S, Hamakado T, Hatanaka M

机构信息

Institute for Virus Research, Kyoto University, Japan.

出版信息

Mol Cell Probes. 1988 Mar;2(1):39-46. doi: 10.1016/0890-8508(88)90042-4.

DOI:10.1016/0890-8508(88)90042-4

PMID:2898099

Abstract

The env-pX IV fused gene of human T-cell leukemia virus type I (HTLV-I) was inserted into lac promoter-directed expression vectors for production of viral proteins in bacteria. Resulting recombinant plasmids, pK13 and pK15, directed synthesis of fused proteins of 59 kDa (Env-p40x) and 100 kDa (Gag-Env-p40x), respectively. Western blot analysis showed that these proteins were reactive with sera of patients with adult T-cell leukemia (ATL) and retained multiple antigenic determinants of viral proteins. In combination with recombinant Gag protein [S. Itamura, K. Shigesada, M. Imai, N. Kobayashi, T. Hamakado, T. Harada and M. Hatanaka, Gene 38, 57-64 (1985)], these bacterially synthesized proteins may provide a useful tool for differential diagnosis of ATL by detecting serum antibodies against individual viral proteins and for analysis of viral gene functions.

摘要

将I型人类T细胞白血病病毒（HTLV-I）的env-pX IV融合基因插入到受lac启动子调控的表达载体中，以便在细菌中生产病毒蛋白。所得到的重组质粒pK13和pK15分别指导合成59 kDa（Env-p40x）和100 kDa（Gag-Env-p40x）的融合蛋白。蛋白质免疫印迹分析表明，这些蛋白能与成人T细胞白血病（ATL）患者的血清发生反应，并保留了病毒蛋白的多个抗原决定簇。与重组Gag蛋白[板村幸男、重贞胜、今井正、小林直、滨加户彻、原田彻和畑中雅，《基因》38卷，57 - 64页（1985年）]相结合，这些细菌合成的蛋白可能为通过检测针对单个病毒蛋白的血清抗体对ATL进行鉴别诊断以及分析病毒基因功能提供一种有用的工具。