Kiyokawa T, Yoshikura H, Hattori S, Seiki M, Yoshida M
Proc Natl Acad Sci U S A. 1984 Oct;81(19):6202-6. doi: 10.1073/pnas.81.19.6202.
The DNA fragments of the 5' and 3' halves of the putative env gene predicted from the DNA sequence of human T-cell leukemia virus (HTLV) provirus were inserted into expression vectors pORF2 and pORF1, respectively, and two hybrid proteins composed of env polypeptides and beta-galactosidase were efficiently produced in Escherichia coli. The hybrid proteins containing the NH2-terminal (EH9) and COOH-terminal (EA1) halves were both immunologically reactive with sera from adult T-cell leukemia patients, demonstrating the utility of the hybrid proteins for diagnosis of HTLV infection. Rabbit antisera against these hybrid proteins detected the two glycoproteins gp62 and gp46, which were previously identified as HTLV env gene products. With these rabbit antisera, two properties of the env gene products were studied. (i) The antisera inhibited syncytia formation of cat S+L- cells induced by HTLV, suggesting that one or both of the env gene products of HTLV, gp62 and gp46, are involved in induction of cell fusion. (ii) The env product gp62 or gp46 or both products are exposed on the surface of HTLV-infected cells and might modulate the proliferation of HTLV-infected T cells in the host because the antisera against the hybrid proteins were cytotoxic on HTLV-producing cell lines. The latter conclusion also is supported by the fact that adult T-cell leukemia patients and healthy HTLV carriers have antibodies to the env gene products.
从人类T细胞白血病病毒（HTLV）前病毒DNA序列预测的假定env基因5'和3'端的DNA片段，分别插入表达载体pORF2和pORF1中，两种由env多肽和β-半乳糖苷酶组成的杂合蛋白在大肠杆菌中高效产生。含有氨基末端（EH9）和羧基末端（EA1）片段的杂合蛋白均与成人T细胞白血病患者的血清发生免疫反应，证明了杂合蛋白在诊断HTLV感染中的实用性。针对这些杂合蛋白的兔抗血清检测到两种糖蛋白gp62和gp46，它们先前被鉴定为HTLV env基因产物。利用这些兔抗血清，研究了env基因产物的两个特性。（i）抗血清抑制了HTLV诱导的猫S+L-细胞的合胞体形成，表明HTLV的env基因产物gp62和gp46中的一种或两种参与了细胞融合的诱导。（ii）env产物gp62或gp46或两者都暴露在HTLV感染细胞的表面，可能调节宿主中HTLV感染T细胞的增殖，因为针对杂合蛋白的抗血清对产生HTLV的细胞系具有细胞毒性。成人T细胞白血病患者和健康的HTLV携带者对env基因产物有抗体这一事实也支持了后一个结论。
