Dental Materials Laboratory, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil; School of Dentistry, University of Federal District, Brasilia, DF, Brazil.
Pharmacology and Therapeutic - Department of Pharmacology, ICBS, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Arch Oral Biol. 2017 Dec;84:106-111. doi: 10.1016/j.archoralbio.2017.09.016. Epub 2017 Sep 25.
To analyze the anti-inflammatory and analgesic effects of an adhesive resin containing indomethacin-loaded nanocapsules in rat model.
Adhesive resin disks with or without indomethacin-loaded nanocapsules were subcutaneously implanted into right hind paw of rats. A week after surgical procedure, 2% formalin solution was intradermally injected into plantar surface of paw. Nociceptive and inflammatory responses were evaluated by formalin test. Paw edema by pletismometer and mechanical hyperalgesia by von Frey test were performed on day 2, day 4, day 6, day 8, day 10 and day 12 after surgery. IL-6, IL-10, and lactate dehydrogenase (LDH) serum levels were determined by ELISA-sandwich test.
Group containing indomethacin-loaded nanocapsules (NC) presented lower edema in the right hind paw at 24h after formalin injection than those of the control group (CT) (P<0.01). NC group showed decrease in the nociceptive response in phase I (neurogenic pain) compared to CT group (NC - 66.86±22.83s X CT - 130.17±35.83s, P<0.001). NC group presented supporting higher intensity of stimulus on days 8 and 12 (24h and 72h after formalin injection) (P<0.01 and P<0.02 respectively). The IL-6 serum level was also significantly higher in the NC group than CT group (p<0.001).
These results indicate that an adhesive resin containing indomethacin-loaded nanocapsules has anti-inflammatory and nociceptive activities in a chemical model of acute inflammation. The present investigation confirms an adhesive resin with drug-loaded nanocapsules may be useful for improving therapeutic effect for adhesives to be used in deep cavities.
分析载吲哚美辛纳米囊的黏附性树脂在大鼠模型中的抗炎和镇痛作用。
将载有或不载吲哚美辛纳米囊的黏附性树脂圆盘皮下植入大鼠右后爪。手术后一周，将 2%福马林溶液皮内注射到足底表面。通过福尔马林试验评估伤害性和炎症反应。在手术后第 2、4、6、8、10 和 12 天，使用 plethysmometer 测量爪肿胀和 von Frey 试验测量机械性痛觉过敏。通过 ELISA 夹心试验测定血清白细胞介素 6（IL-6）、白细胞介素 10（IL-10）和乳酸脱氢酶（LDH）水平。
载吲哚美辛纳米囊组（NC 组）在福马林注射后 24 小时，右后爪肿胀程度低于对照组（CT 组）（P<0.01）。与 CT 组相比，NC 组在相 I（神经源性疼痛）中的疼痛反应减少（NC - 66.86±22.83s X CT - 130.17±35.83s，P<0.001）。NC 组在第 8 天和第 12 天（福马林注射后 24 小时和 72 小时）对刺激的强度更高（P<0.01 和 P<0.02）。NC 组的血清 IL-6 水平也明显高于 CT 组（p<0.001）。
这些结果表明，载吲哚美辛纳米囊的黏附性树脂在化学诱导的急性炎症模型中具有抗炎和镇痛活性。本研究证实，载药纳米囊的黏附性树脂可能有助于提高用于深腔的黏附剂的治疗效果。
