Genari Bruna, Leitune Vicente Castelo Branco, Jornada Denise Soledade, Camassola Melissa, Arthur Rodrigo Alex, Pohlmann Adriana Raffin, Guterres Silvia Stanisçuaski, Collares Fabrício Mezzomo, Samuel Susana Maria Werner
Dental Materials Laboratory, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Pharmaceutical Sciences, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
Dent Mater. 2017 Jun;33(6):735-742. doi: 10.1016/j.dental.2017.04.001. Epub 2017 Apr 24.
To incorporate indomethacin and triclosan-loaded nanocapsules into primer and adhesive, and evaluate its properties.
Indomethacin and triclosan were encapsulated by deposition of preformed polymer and subsequently characterized regarding morphology, particle size, drug content and cytotoxicity. Nanocapsules (NCs) were incorporated into primer at 2% and into adhesive at 1, 2, 5, and 10% concentrations. Degree of conversion (DC) and softening in ethanol of the adhesive were evaluated. Drug release and drug diffusion through dentin was quantified by high performance liquid chromatography. Antimicrobial test was performed until 96h.
Spherical and biocompatible NCs presented mean size of 159nm. Drugs content was 3mg indomethacin/g powder and 2mg triclosan/g powder. Incorporating NCs in adhesive showed no influence in DC (p=0.335). The addition of 2% of NCs showed no influence in softening in ethanol (p>0.05). After 120h, 93% of indomethacin and 80% of triclosan were released from primer, 20% of indomethacin and 17% of triclosan were released from adhesive with 10% of NCs. Indomethacin showed diffusion through dentin. In 24h, adhesive containing 2 and 5% of NCs using primer with NCs showed antimicrobial effect. In 96h, adhesives containing different concentration of NCs promoted antimicrobial effect.
Indomethacin and triclosan-loaded nanocapsules were successfully incorporated into primer and adhesive, promoting controlled drugs release, indomethacin diffusion through dentin and antimicrobial effect without compromising its physicochemical properties.
Indomethacin and triclosan-loaded nanocapsules have potential to prevent recurrent caries and to be used in deep cavities controlling pulpar inflammatory process.
将载有吲哚美辛和三氯生的纳米胶囊掺入底漆和粘合剂中，并评估其性能。
通过预制聚合物的沉积来包封吲哚美辛和三氯生，随后对其形态、粒径、药物含量和细胞毒性进行表征。将纳米胶囊（NCs）以2%的浓度掺入底漆中，并以1%、2%、5%和10%的浓度掺入粘合剂中。评估粘合剂在乙醇中的转化率（DC）和软化情况。通过高效液相色谱法定量药物释放和药物通过牙本质的扩散。进行96小时的抗菌测试。
球形且具有生物相容性的NCs平均粒径为159nm。药物含量为每克粉末含3mg吲哚美辛和2mg三氯生。在粘合剂中掺入NCs对DC无影响（p=0.335）。添加2%的NCs对在乙醇中的软化无影响（p>0.05）。120小时后，93%的吲哚美辛和80%的三氯生从底漆中释放出来，20%的吲哚美辛和17%的三氯生从含有10%NCs的粘合剂中释放出来。吲哚美辛显示出通过牙本质的扩散。在24小时内，使用含NCs底漆且含有2%和5%NCs的粘合剂显示出抗菌效果。在96小时内，含有不同浓度NCs的粘合剂均促进了抗菌效果。
载有吲哚美辛和三氯生的纳米胶囊成功掺入底漆和粘合剂中，促进了药物的控释、吲哚美辛通过牙本质的扩散以及抗菌效果，同时不影响其物理化学性质。
载有吲哚美辛和三氯生的纳米胶囊有预防复发性龋齿以及用于深龋控制牙髓炎症过程的潜力。
