Ingerslev Anne Krog, Mutt Shivaprakash Jagalur, Lærke Helle Nygaard, Hedemann Mette Skou, Theil Peter Kappel, Nielsen Kirstine Lykke, Jørgensen Henry, Herzig Karl-Heinz, Bach Knudsen Knud Erik
Department of Animal Science, Aarhus University, Tjele, Denmark.
Research Unit of Biomedicine and Biocenter of Oulu, Department of Physiology, University of Oulu, Oulu, Finland.
PLoS One. 2017 Oct 5;12(10):e0185927. doi: 10.1371/journal.pone.0185927. eCollection 2017.
Increased dietary fiber (DF) fermentation and short-chain fatty acid (SCFA) production may stimulate peptide tyrosine-tyrosine (PYY) secretion. In this study, the effects of hindgut SCFA production on postprandial PYY plasma levels were assessed using different experimental diets in a porto-arterial catheterized pig model. The pigs were fed experimental diets varying in source and levels of DF for one week in 3×3 Latin square designs. The DF sources were whole-wheat grain, wheat aleurone, rye aleurone-rich flour, rye flakes, and resistant starch. Postprandial blood samples were collected from the catheters and analyzed for PYY levels and net portal appearance (NPA) of PYY was correlated to NPA of SCFA. No significant effects of diets on NPA of PYY were observed (P > 0.05), however, resistant starch supplementation increased postprandial NPA of PYY levels by 37 to 54% compared with rye-based and Western-style control diets (P = 0.19). This increase was caused by higher mesenteric artery and portal vein PYY plasma levels (P < 0.001) and was independent of SCFA absorption (P > 0.05). The PYY levels were higher in response to the second daily meal compared with the first daily meal (P < 0.001), but similar among diets (P > 0.10). In conclusion, the increased postprandial PYY responses in pigs fed with different levels and sources of DF are not caused by an increased SCFA absorption and suggest that other mechanisms such as neural reflexes and possibly an increased flow of digesta in the small intestine may be involved. The content of DF and SCFA production did not affect PYY levels.
膳食纤维（DF）发酵增加和短链脂肪酸（SCFA）生成增多可能会刺激肽酪酪肽（PYY）的分泌。在本研究中，在采用动脉导管插入术的猪模型中，使用不同的实验性日粮评估后肠SCFA生成对餐后血浆PYY水平的影响。在3×3拉丁方设计中，让猪连续一周采食DF来源和水平不同的实验性日粮。DF来源包括全麦谷物、小麦糊粉层、富含黑麦糊粉层的面粉、黑麦片和抗性淀粉。从导管采集餐后血样，分析PYY水平，并且将PYY的净门静脉出现量（NPA）与SCFA的NPA进行关联分析。未观察到日粮对PYY的NPA有显著影响（P>0.05），然而，与基于黑麦的日粮和西式对照日粮相比，补充抗性淀粉使餐后PYY水平的NPA提高了37%至54%（P=0.19）。这种升高是由肠系膜动脉和门静脉中PYY血浆水平较高所致（P<0.001），并且与SCFA吸收无关（P>0.05）。与第一餐相比，第二餐进食后PYY水平更高（P<0.001），但不同日粮之间的PYY水平相似（P>0.10）。总之，采食不同水平和来源DF的猪餐后PYY反应增加并非由SCFA吸收增加所致，这表明可能涉及其他机制，如神经反射以及可能小肠中食糜流动增加。DF含量和SCFA生成并未影响PYY水平。
