Moon T D
Tulane University, New Orleans, LA.
Biochem Biophys Res Commun. 1988 Jun 16;153(2):722-7. doi: 10.1016/s0006-291x(88)81154-9.
The effect of opiate receptor agonists upon cell growth of the prostatic carcinoma cell line DU145 were studied. Dynorphin-A increased growth significantly with a peak response at 10(-13) M, of 21 +/- 4% (mean +/- SEM). The dose response curve had a typical inverted-U shape. Dynorphin fragments 1-13 and 1-7 also increased growth at 10(-13) M, while the 2-13 fragment failed to increase growth. Naloxone increased growth at high concentration (10(-7) M) suggesting a stimulatory effect, while at the same time blocking the effect of dynorphin-A. This data demonstrates that agents which stimulate opiate receptors, especially the kappa receptor agonist dynorphin, increase the growth of prostatic carcinoma, and that this effect is controlled by changes at the N-terminal end of the peptide. This effect is blocked by Naloxone.
研究了阿片受体激动剂对前列腺癌细胞系DU145细胞生长的影响。强啡肽A显著促进生长，在10(-13)M时出现峰值反应，为21±4%（平均值±标准误）。剂量反应曲线呈典型的倒U形。强啡肽片段1-13和1-7在10(-13)M时也促进生长，而2-13片段未能促进生长。纳洛酮在高浓度（10(-7)M）时促进生长，表明有刺激作用，同时阻断强啡肽A的作用。该数据表明，刺激阿片受体的药物，尤其是κ受体激动剂强啡肽，可促进前列腺癌生长，且这种作用受肽N端变化的控制。这种作用被纳洛酮阻断。
