Mastronikolis Nicholas S, Tsiambas Evangelos, Papadas Theodoros A, Karameris Andreas, Ragos Vasileios, Peschos Dimitrios, Mastronikolis Stylianos N, Papadas Athanasios T, Liatsos Christos, Armata Ilianna E, Fotiades Panagiotis P
ENT Department, Medical School, University of Patras, Patras, Greece.
Department of Immunohistochemistry & Molecular Biology, 401 GAH, Athens, Greece
Anticancer Res. 2017 Oct;37(10):5521-5524. doi: 10.21873/anticanres.11983.
BACKGROUND/AIM: Phosphatase and tensin homolog (PTEN) (gene locus: 10q23.3) -a tumor suppressor gene- is deleted, mutated or epigenetically hyper-methylated in a variety of malignancies. PTEN acts as a negative regulator in PI3K/AKT/mTOR signaling transduction pathway. Our aim was to investigate PTEN protein expression patterns in laryngeal squamous cell carcinomas (LSCC).
Using tissue microarray technology, fifty (n=50) primary LSCCs were cored and re-embedded into one recipient block. Immunohistochemistry and digital image analysis were implemented for evaluating protein expression levels.
Abnormal protein expression (low to negative staining intensity values) was observed in 28/50 (56%) tissue cores. Overall PTEN expression was associated with the anatomical region of the malignancies (p=0.039), whereas a borderline correlation with the differentiation grade was also assessed (p=0.05).
Aberrant expression of PTEN tumor-suppressor gene in LSCCs seems to affect their biological behavior. Well-differentiated tumors express moderate to high protein levels, an evidence of normal gene function, whereas loss of its expression correlates with a progressive tumor dedifferentiation. Additionally, loss of its expression is detected more frequently in specific anatomical regions of the larynx (glottis, predominantly, and partially supraglottis).
背景/目的：磷酸酶和张力蛋白同源物（PTEN）（基因座：10q23.3）——一种肿瘤抑制基因——在多种恶性肿瘤中存在缺失、突变或表观遗传高甲基化。PTEN在PI3K/AKT/mTOR信号转导通路中起负调节作用。我们的目的是研究喉鳞状细胞癌（LSCC）中PTEN蛋白的表达模式。
采用组织芯片技术，从50例原发性LSCC中获取组织芯并重新包埋于一个受体块中。采用免疫组织化学和数字图像分析评估蛋白表达水平。
在50个组织芯中的28个（56%）观察到异常蛋白表达（低至阴性染色强度值）。总体PTEN表达与恶性肿瘤的解剖区域相关（p = 0.039），同时也评估了与分化程度的临界相关性（p = 0.05）。
LSCC中PTEN肿瘤抑制基因的异常表达似乎影响其生物学行为。高分化肿瘤表达中等至高蛋白质水平，这是基因功能正常的证据，而其表达缺失与肿瘤的逐渐去分化相关。此外，在喉的特定解剖区域（主要是声门和部分声门上区）更频繁地检测到其表达缺失。
