Liu Jun, Lei Da-Peng, Jin Tong, Zhao Xue-Ning, Li Guojun, Pan Xin-Liang
Department of Otolaryngology, Qilu Hospital, Shandong University, Jinan, China.
Asian Pac J Cancer Prev. 2011;12(10):2653-7.
The purpose of this study was to examine the expression of mir-21 and phosphatase and tensin homologue (PTEN) in laryngeal squamous cell carcinomas (LSCCs) and hypopharyngeal squamous cell carcinomas (HSCCs), and assess correlations between the two as well as with clinical characteristics of patients.
The expression of mir-21 in tumor and adjacent non-tumor tissues was investigated by real-time RT-PCR. Immunohistochemistry (IHC) was carried out to analyze PTEN protein levels.
Mir-21 was up-regulated in LSCCs and HSCCs compared to adjacent non-tumor tissues (P < 0.05), and the up-regulated expression of mir-21 was associated with clinical stage (P = 0.001), T classification (P = 0.007), pathologic differentiation (P = 0.025), and lymph node positivity (P = 0.002). In contrast, PTEN IHC staining was notably weaker in tumor tissues than in matched non-tumor tissues (P < 0.05), and the down-regulated expression of PTEN was correlated with tumor staging (P = 0.025), the extent of tumor (P = 0.017), and lymph node positivity (P = 0.040). Furthermore, the level of mir-21 was reversely correlated with PTEN expression (P = 0.006).
mir-21 and PTEN might play important roles in the progression of LSCC and HSCC, the two fcators demonstrating a negative correlation.
本研究旨在检测微小RNA-21（mir-21）和磷酸酶及张力蛋白同源物（PTEN）在喉鳞状细胞癌（LSCC）和下咽鳞状细胞癌（HSCC）中的表达，并评估二者之间的相关性以及与患者临床特征的相关性。
采用实时逆转录聚合酶链反应（RT-PCR）检测肿瘤组织及癌旁非肿瘤组织中mir-21的表达。采用免疫组织化学（IHC）分析PTEN蛋白水平。
与癌旁非肿瘤组织相比，LSCC和HSCC中mir-21表达上调（P<0.05），且mir-21表达上调与临床分期（P=0.001）、T分级（P=0.007）、病理分化（P=0.025）及淋巴结阳性（P=0.002）相关。相反，肿瘤组织中PTEN免疫组化染色明显弱于配对的非肿瘤组织（P<0.05），PTEN表达下调与肿瘤分期（P=0.025）、肿瘤范围（P=0.017）及淋巴结阳性（P=0.040）相关。此外，mir-21水平与PTEN表达呈负相关（P=0.006）。
mir-21和PTEN可能在LSCC和HSCC进展中起重要作用，二者呈负相关。
