Mesguich P, Benoit R, Dubois P M, Morel G
CNRS UA 559, Laboratoire d'Histologie-Embryologie, Faculté de Médecine Lyon-Sud, Oullins, France.
Cell Tissue Res. 1988 May;252(2):419-27. doi: 10.1007/BF00214385.
Endogenous SS14- as well as SS28-like immunoreactive materials were detected in both male and female rats by radioimmunoassay and by immunocytochemistry on ultrathin frozen sections. The content of somatostatin-like immunoreactivity was 0.39 +/- 0.08 pg per mg adenohypophysis. Immunoreactivity was localized by immunocytochemistry in three pituitary cell types: somatotrophs, lactotrophs and thyrotrophs, but not in corticotrophs and gonadotrophs. In these three pituitary cell types the SS28- and the SS14-like immunoreactive materials were localized in the cytoplasm and in the nucleus. In the cytoplasm the immunoreactivity was seen in the cytoplasmic matrix and in the secretory granules. In the nucleus it was present mainly in the euchromatin close to the heterochromatin. In somatotrophs and lactotrophs, SS14- and SS28-like immunoreactive materials have been detected at the plasma membrane level. These results suggest that (1) endogenous SS14 and SS28 are present in adenohypophysis in somatotrophs, lactotrophs and thyrotrophs, and (2) the two peptides act on both the cytoplasmic components and the nucleus.
通过放射免疫分析法以及对超薄冰冻切片进行免疫细胞化学检测，在雄性和雌性大鼠中均检测到了内源性SS14以及类似SS28的免疫反应性物质。促生长激素细胞、促乳素细胞和促甲状腺激素细胞中生长抑素样免疫反应性的含量为每毫克腺垂体0.39±0.08皮克。通过免疫细胞化学方法，免疫反应性定位于三种垂体细胞类型中：促生长激素细胞、促乳素细胞和促甲状腺激素细胞，但在促肾上腺皮质激素细胞和促性腺激素细胞中未检测到。在这三种垂体细胞类型中，类似SS28和SS14的免疫反应性物质定位于细胞质和细胞核中。在细胞质中，免疫反应性可见于细胞质基质和分泌颗粒中。在细胞核中，它主要存在于靠近异染色质的常染色质中。在促生长激素细胞和促乳素细胞中，已在质膜水平检测到类似SS14和SS28的免疫反应性物质。这些结果表明：（1）腺垂体的促生长激素细胞、促乳素细胞和促甲状腺激素细胞中存在内源性SS14和SS28；（2）这两种肽作用于细胞质成分和细胞核。
