Bakhit C, Koda L, Benoit R, Morrison J H, Bloom F E
J Neurosci. 1984 Feb;4(2):411-9. doi: 10.1523/JNEUROSCI.04-02-00411.1984.
Cysteamine administration to rats results in a marked depletion of hypothalamic somatostatin-14 (SS14) and a decrease of the potassium-evoked in vitro release of SS14 without a significant change in the content or release of somatostatin-28(1-12)-like immunoreactivity (SS28(1-12)-L1). Furthermore, cysteamine enhances the spontaneous release and markedly potentiates the potassium-evoked release of SS14 in the in vitro slice preparation. However, in vitro-administered cysteamine does not alter the spontaneous or potassium-evoked release of SS28(1-12)-LI. Immunohistochemical visualization of hypothalamic neuronal cell bodies and fibers following cysteamine administration shows a disappearance of the SS14 immunoreactive fibers and cell bodies with no apparent change in the SS28(1-12) immunoreactive fibers and cell bodies. These data suggest that, in rat hypothalamus, selective release of SS14 and SS28(1-12) can occur. The results are discussed in relation to possible sites of storage and release of the somatostatin-related peptides from synaptic nerve terminals.
给大鼠施用半胱胺会导致下丘脑生长抑素 - 14（SS14）显著减少，并且在体外，钾离子诱发的SS14释放量降低，而生长抑素 - 28（1 - 12）样免疫反应性物质（SS28(1 - 12)-LI）的含量或释放量无显著变化。此外，在体外脑片制备中，半胱胺可增强SS14的自发释放，并显著增强钾离子诱发的SS14释放。然而，体外施用半胱胺不会改变SS28(1 - 12)-LI的自发释放或钾离子诱发的释放。半胱胺给药后下丘脑神经元细胞体和纤维的免疫组织化学观察显示，SS14免疫反应性纤维和细胞体消失，而SS28(1 - 12)免疫反应性纤维和细胞体无明显变化。这些数据表明，在大鼠下丘脑中，SS14和SS28(1 - 12)可能会选择性释放。文中结合生长抑素相关肽从突触神经末梢储存和释放的可能位点对结果进行了讨论。
