Tincher Clayton, Long Hongan, Behringer Megan, Walker Noah, Lynch Michael
Department of Biology, Indiana University, Bloomington, Indiana 47405.
Department of Biology, Indiana University, Bloomington, Indiana 47405
G3 (Bethesda). 2017 Oct 5;7(10):3331-3335. doi: 10.1534/g3.117.300133.
Mutations induced by pollutants may promote pathogen evolution, for example by accelerating mutations conferring antibiotic resistance. Generally, evaluating the genome-wide mutagenic effects of long-term sublethal pollutant exposure at single-nucleotide resolution is extremely difficult. To overcome this technical barrier, we use the mutation accumulation/whole-genome sequencing (MA/WGS) method as a mutagenicity test, to quantitatively evaluate genome-wide mutagenesis of after long-term exposure to a wide gradient of the glyphosate-based herbicide (GBH) Roundup Concentrate Plus. The genome-wide mutation rate decreases as GBH concentration increases, suggesting that even long-term GBH exposure does not compromise the genome stability of bacteria.
污染物诱导的突变可能会促进病原体进化，例如通过加速赋予抗生素抗性的突变。一般来说，在单核苷酸分辨率下评估长期亚致死性污染物暴露的全基因组诱变效应极其困难。为了克服这一技术障碍，我们使用突变积累/全基因组测序（MA/WGS）方法作为一种诱变性测试，以定量评估长期暴露于不同梯度的草甘膦基除草剂（GBH）“农达强化剂”后全基因组的诱变情况。全基因组突变率随着GBH浓度的增加而降低，这表明即使长期暴露于GBH也不会损害细菌的基因组稳定性。
