Kunz W, Gellerich F N, Schild L, Schönfeld P
Institut für Biochemie, Medizinische Akademie Magdeburg, GDR.
FEBS Lett. 1988 Jun 6;233(1):17-21. doi: 10.1016/0014-5793(88)81347-4.
Changes in J0, delta muH+ and delta GexP were investigated as a function of load. The flux control coefficients, particularly those of the adenine nucleotide translocator and H+-ATPase at the maximum rate of oxidative phosphorylation were seen to strongly depend on the phosphate concentration accounting in common for the highest share in flux control. There was no unique relationship observed between JP and delta muH+ in load-controlled, well coupled systems, but JP was found to depend on delta muH+ at excessive load and increasing proton leakage. All the results presented can be elucidated on the grounds of delocalized chemiosmotic coupling.
研究了 J0、ΔμH⁺ 和 ΔGexP 随负荷的变化。通量控制系数，尤其是在氧化磷酸化最大速率时腺嘌呤核苷酸转位酶和 H⁺ -ATP 酶的通量控制系数，被发现强烈依赖于磷酸盐浓度，磷酸盐浓度在通量控制中占最大份额。在负荷控制、耦合良好的系统中，未观察到 JP 和 ΔμH⁺ 之间存在独特关系，但发现在负荷过大和质子泄漏增加时，JP 依赖于 ΔμH⁺。所呈现的所有结果都可以基于离域化学渗透耦合来解释。
