Semnani Mohammadreza, Mashayekhi Farhad, Azarnia Mahnaz, Salehi Zivar
Folia Neuropathol. 2017;55(3):199-205. doi: 10.5114/fn.2017.70484.
The cuprizone multiple sclerosis (MS) animal model is characteristic for toxic demyelination and represents a reversible demyelination and remyelination system. It has been shown that green tea epigallocatechin-3-gallate (EGCG) might be effective in improving the symptoms and pathological conditions associated with autoimmune inflammatory diseases in several animal models. In this study the effects of EGCG on proteolipid protein (PLP) and oligodendrocyte transcription factor 1 (Olig1) expression in the cerebral cortex of a murine model of cuprizone-induced demyelination was investigated. C57BL/6 mice were treated with cuprizone for six weeks in order to induce demyelination. Immediately after the cessation of cuprizone the animals were divided into 6 groups (n = 10 for each group). The first two groups were injected intraperitoneally (IP) with EGCG in the amount of 50 mg/kg/daily body weight for 2 and 4 weeks. The second two groups (SHAM) were injected IP with phosphate-buffered saline (PBS) for 2 and 4 weeks, and the third two groups were left without injection as controls. After two and four weeks the mice were killed and the cerebral cortex was collected and the expression of Plp and Olig1 was studied by real-time PCR. The results showed significant increases in PLP and Olig1 expression in the EGCG-treated groups as compared to the SHAM and control groups (p < 0.0001). It is concluded that EGCG increases PLP and Olig1 expression in the cerebral cortex of a mouse model of MS induced by cuprizone.
铜螯合剂多发性硬化症（MS）动物模型具有毒性脱髓鞘的特征，是一种可逆的脱髓鞘和髓鞘再生系统。研究表明，在几种动物模型中，绿茶表没食子儿茶素-3-没食子酸酯（EGCG）可能对改善自身免疫性炎症疾病相关的症状和病理状况有效。本研究调查了EGCG对铜螯合剂诱导的脱髓鞘小鼠模型大脑皮质中蛋白脂蛋白（PLP）和少突胶质细胞转录因子1（Olig1）表达的影响。C57BL/6小鼠用铜螯合剂处理六周以诱导脱髓鞘。停止使用铜螯合剂后，立即将动物分为6组（每组n = 10）。前两组腹腔注射（IP）50 mg/kg/每日体重的EGCG，持续2周和4周。后两组（假手术组）腹腔注射磷酸盐缓冲盐水（PBS），持续2周和4周，第三组两组不注射作为对照。2周和4周后，处死小鼠并收集大脑皮质，通过实时PCR研究Plp和Olig1的表达。结果显示，与假手术组和对照组相比，EGCG处理组的PLP和Olig1表达显著增加（p < 0.0001）。得出结论，EGCG可增加铜螯合剂诱导的MS小鼠模型大脑皮质中PLP和Olig1的表达。
