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DNA条形码技术在幼体后阶段可增进对鱼类生物多样性的了解:以西南印度洋留尼汪岛为例。

DNA barcoding post-larvae can improve the knowledge about fish biodiversity: an example from La Reunion, SW Indian Ocean.

作者信息

Collet Adeline, Durand Jean-Dominique, Desmarais Eric, Cerqueira Frédérique, Cantinelli Thomas, Valade Pierre, Ponton Dominique

机构信息

a OCEA Consult' , La Réunion , France.

b UMR 9190 MARBEC, IRD-CNRS-IFREMER-UM, Department of Ecology , Evolutionary Biology University of Science, VNUHCM , Ho Chi Minh City , Vietnam.

出版信息

Mitochondrial DNA A DNA Mapp Seq Anal. 2018 Aug;29(6):905-918. doi: 10.1080/24701394.2017.1383406. Epub 2017 Oct 6.

DOI:10.1080/24701394.2017.1383406
PMID:28984152
Abstract

The aim of this study was to demonstrate that fish larvae identified using their COI sequences offer a unique opportunity for improving the knowledge of local fish richness. Fish larvae were sampled at the end of their pelagic phase using light-traps set off the West Coast of La Reunion Island, southwestern Indian Ocean, once per month from October 2014 to March 2015. Among the 5174 larvae caught, 214 morphologically different specimens were selected, 196 successfully barcoded, giving a total of 101 different Barcode Index Numbers (BINs). Among these BINs, 55 had never been recorded in La Reunion exclusive economic zone (EEZ), and 13 were new for the BOLD database. Even if the sampling effort for collecting fish post-larvae during this study was relatively low, it allowed adding at least nine new species to an updated checklist of fishes of La Reunion EEZ.

摘要

本研究的目的是证明,利用细胞色素氧化酶亚基I(COI)序列鉴定的鱼类幼体为增进对当地鱼类丰富度的了解提供了独特机会。2014年10月至2015年3月期间,每月一次在印度洋西南部留尼汪岛西海岸外设置的灯光诱捕器在鱼类幼体的浮游阶段结束时对其进行采样。在所捕获的5174尾幼体中,挑选出214个形态各异的标本,其中196个成功进行了条形码编码,共得到101个不同的条形码索引号(BINs)。在这些BINs中,有55个在留尼汪专属经济区(EEZ)从未有过记录,13个对于生物条形码数据库(BOLD)来说是新的。即使在本研究中收集鱼类后期幼体的采样工作量相对较低,但它至少能为留尼汪专属经济区鱼类的更新清单增加9个新物种。

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