Shaked I, Sod-Moriah U A, Kaplanski J, Potashnik G, Buchman O
Department of Biology, Faculty of Health Sciences, Ben Gurion University of the Negev, Beer Sheva, Israel.
Int J Fertil. 1988 Mar-Apr;33(2):129-33.
Dibromochloropropane (DBCP) is an effective nematocide which has been shown to suppress spermatogenesis and cause infertility in both men and male rats. There are no similar reports concerning the effects of DBCP on female reproduction. The purpose of the present study was to attempt to interfere with the various phases of oogenesis. Proestral or pregnant rats were injected subcutaneously once with 40 mg/kg DBCP on one of each days of L12-L20 of gestation; a double dose (80 mg/kg) was injected in eight consecutive days (L11-L18). In addition, L13 fetuses were injected--directly into the amniotic sac--with 0.1 mg DBCP. Pooled data from the various days of gestation revealed that postimplantation losses were three times as high in the DBCP-treated animals as in DMSO-treated controls. Perinatal deaths were 58% higher and mean pup weights were 30% lower in the DBCP-treated rats than in controls. The reproductive performance of females exposed to DBCP while in utero was affected only to a limited degree (reduced number of ovulations and implantations) as compared with their DMSO counterparts. Doubling the dose (80 mg/kg) seriously reduced the birth weight of pups (50% of controls), all of which died within several hours post-partum. Direct injection of DBCP into embryos or to proestral rats did not have any adverse effects on their future reproductive performance. In contrast to the effect on spermatogenesis, it appears that oogenesis and ova are unaffected by DBCP.
二溴氯丙烷（DBCP）是一种有效的杀线虫剂，已被证明会抑制精子发生，并导致男性和雄性大鼠不育。关于DBCP对雌性生殖影响的类似报道尚未见。本研究的目的是试图干扰卵子发生的各个阶段。在妊娠L12 - L20的每一天，对动情前期或怀孕的大鼠皮下注射一次40mg/kg DBCP；连续八天（L11 - L18）注射双倍剂量（80mg/kg）。此外，对L13期胎儿直接向羊膜腔内注射0.1mg DBCP。来自不同妊娠天数的汇总数据显示，DBCP处理组动物的着床后损失是二甲基亚砜（DMSO）处理对照组的三倍。与对照组相比，DBCP处理组大鼠的围产期死亡率高58%，平均幼崽体重低30%。与DMSO处理的雌性相比，子宫内接触DBCP的雌性生殖性能仅受到有限程度的影响（排卵和着床数量减少）。将剂量加倍（80mg/kg）会严重降低幼崽的出生体重（为对照组的50%），所有幼崽在产后数小时内死亡。将DBCP直接注射到胚胎或动情前期大鼠体内对其未来的生殖性能没有任何不利影响。与对精子发生的影响相反，卵子发生和卵子似乎不受DBCP的影响。
