Sheu Shih-Yi, Xie Pei-Bei, Sheu Ceshing, Chen Wen-Ming
Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Department of Applied Chemistry, Chaoyang University of Technology, No.168, Jifong E. Rd., Wufeng, Taichung, Taiwan, ROC.
Int J Syst Evol Microbiol. 2017 Nov;67(11):4747-4753. doi: 10.1099/ijsem.0.002371. Epub 2017 Oct 6.
A bacterial strain designated LSN-49 was isolated from a brackish river in Taiwan and characterized using a polyphasic taxonomy approach. Cells of strain LSN-49 were Gram-staining-negative, aerobic, poly-β-hydroxybutyrate accumulating, motile by means of a monopolar flagellum, non-spore forming, straight rods and formed shiny and translucent colonies. Growth occurred at 20-40 °C (optimum, 25-30 °C), at pH 6-10 (optimum, pH 7-8) and with 0-3 % (w/v) NaCl [optimum, 0-1 % (w/v)]. The predominant fatty acids were summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), C17 : 1ω8c and C16 : 0. The polar lipid profile consisted of a mixture of phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine, (PC), two uncharacterized aminophospholipids (APL1 and APL2), one uncharacterized glycolipid (GL1), four uncharacterized phospholipids (PL1-PL4) and four uncharacterized lipids (L1-L4). The major polyamine was putrescine. The major isoprenoid quinone was Q-8 and the DNA G+C content was 51.0 mol%. The results of phylogenetic analyses based on 16S rRNA gene sequences indicated that LSN-49 formed a distinct lineage with respect to closely related genera in the family Pseudoalteromonadaceae. LSN-49 was most closely related to Pseudoalteromonas, Algicola and Psychrosphaera and showed 89.3-92.1 % sequence similarity with members of the family Pseudoalteromonadaceae with validly published names. On the basis of the genotypic and phenotypic data, LSN-49 represents a novel genus and species of the family Pseudoalteromonadaceae, for which the name Salsuginimonas clara gen. nov., sp. nov. is proposed. The type strain is LSN-49 (=BCRC 81005=LMG 29726=KCTC 52439).
从台湾一条微咸河流中分离出一株名为LSN - 49的细菌菌株，并采用多相分类法对其进行了特征描述。LSN - 49菌株细胞革兰氏染色阴性，好氧，能积累聚-β-羟基丁酸，借助单极鞭毛运动，不形成芽孢，为直杆状，形成有光泽且半透明的菌落。在20 - 40 °C（最适温度为25 - 30 °C）、pH值6 - 10（最适pH值为7 - 8）以及0 - 3 %（w/v）NaCl [最适为0 - 1 %（w/v）]的条件下生长。主要脂肪酸为总和特征3（包含C16 : 1ω7c和/或C16 : 1ω6c）、C17 : 1ω8c和C16 : 0。极性脂质谱由磷脂酰乙醇胺（PE）、磷脂酰甘油（PG）、磷脂酰胆碱（PC）、两种未鉴定的氨基磷脂（APL1和APL2）、一种未鉴定的糖脂（GL1）、四种未鉴定的磷脂（PL1 - PL4）和四种未鉴定的脂质（L1 - L4）组成。主要多胺为腐胺。主要类异戊二烯醌为Q - 8，DNA G + C含量为51.0 mol%。基于16S rRNA基因序列的系统发育分析结果表明，LSN - 49相对于假交替单胞菌科中密切相关的属形成了一个独特的谱系。LSN - 49与假交替单胞菌属、嗜冷噬琼胶菌属和嗜冷球胞菌属关系最为密切，与已有效发表名称的假交替单胞菌科成员的序列相似性为89.3 - 92.1 %。基于基因型和表型数据，LSN - 49代表假交替单胞菌科的一个新属和新种，为此提出新属名盐栖单胞菌属（Salsuginimonas）和新种名清澈盐栖单胞菌（Salsuginimonas clara）。模式菌株为LSN - 49（=BCRC 81005=LMG 29726=KCTC 52439）。
