一种人类皮肤癌细胞系分泌的可溶性因子支持成人T细胞白血病细胞的克隆生长。
A soluble-factor(s) secreted by a human skin cancer cell line supports clonal growth of adult T-cell leukemia cells.
作者信息
Kagami Y, Kinoshita T, Shimoyama M, Miwa M
机构信息
Virology Division, National Cancer Center Research Institute, Tokyo.
出版信息
Jpn J Cancer Res. 1988 Apr;79(4):424-7. doi: 10.1111/j.1349-7006.1988.tb01608.x.
Leukemic cells from four out of eight patients with adult T-cell leukemia (ATL) were successfully grown by cocultivation with HSC-I cells, a human skin cancer cell line, in the presence of interleukin-2. Three of these four cultures of growing cells showed rearrangement of the T-cell receptor beta-chain gene like the original leukemic cells in vivo, and also showed conservation of the patterns of HTLV-I integration of the original leukemic cells in vivo. Cell-to-cell contact between HSC-I cells and leukemic cells was not necessary for growth of the leukemic cells. The results indicate that some soluble growth factor secreted by HSC-I cells and interleukin-2 are required for the in vitro growth of leukemic cells from some patients with adult T-cell leukemia.
在白细胞介素-2存在的情况下，通过与人类皮肤癌细胞系HSC-I细胞共培养，成功培养出了8例成人T细胞白血病（ATL）患者中4例患者的白血病细胞。这4种生长细胞培养物中的3种显示出T细胞受体β链基因重排，类似于体内原始白血病细胞，并且还保留了体内原始白血病细胞的HTLV-I整合模式。白血病细胞的生长并不需要HSC-I细胞与白血病细胞之间的细胞间接触。结果表明，HSC-I细胞分泌的一些可溶性生长因子和白细胞介素-2是某些成人T细胞白血病患者白血病细胞体外生长所必需的。
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