快照：组织透明化。

SnapShot: Tissue Clearing.

机构信息

Harvard Center for Biological Imaging, Harvard University, Cambridge, MA 02138, USA; Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.

Harvard Center for Biological Imaging, Harvard University, Cambridge, MA 02138, USA; Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA; Center for Brain Science, Harvard University, Cambridge, MA 02138, USA.

出版信息

Cell. 2017 Oct 5;171(2):496-496.e1. doi: 10.1016/j.cell.2017.09.025.

DOI:10.1016/j.cell.2017.09.025

PMID:28985569

Abstract

Tissue clearing has become an important tool for the investigation of biological systems in three dimensions. However, many pioneering techniques were based on serendipitous discoveries. Next-generation clearing methods have been (re)designed with a better understanding of the chemistry and physics required to equalize the refractive index throughout a sample which prevents the random bending of light that clouds biological tissues.

摘要

组织透明化已成为研究生物系统三维结构的重要工具。然而，许多开创性的技术都是基于偶然的发现。新一代的透明化方法是在更好地理解化学和物理原理的基础上重新设计的，这些原理是为了在整个样本中实现折射率的均匀化，以防止光线的随机弯曲，从而使生物组织变得不清晰。