Marine Biochemical Sciences Group, Chemical Sciences Division, Hollings Marine Laboratory, National Institute of Standards and Technology, Charleston, SC
Statistical Engineering Division, National Institute of Standards and Technology, Gaithersburg, MD.
J Lipid Res. 2017 Dec;58(12):2275-2288. doi: 10.1194/jlr.M079012. Epub 2017 Oct 6.
As the lipidomics field continues to advance, self-evaluation within the community is critical. Here, we performed an interlaboratory comparison exercise for lipidomics using Standard Reference Material (SRM) 1950-Metabolites in Frozen Human Plasma, a commercially available reference material. The interlaboratory study comprised 31 diverse laboratories, with each laboratory using a different lipidomics workflow. A total of 1,527 unique lipids were measured across all laboratories and consensus location estimates and associated uncertainties were determined for 339 of these lipids measured at the sum composition level by five or more participating laboratories. These evaluated lipids detected in SRM 1950 serve as community-wide benchmarks for intra- and interlaboratory quality control and method validation. These analyses were performed using nonstandardized laboratory-independent workflows. The consensus locations were also compared with a previous examination of SRM 1950 by the LIPID MAPS consortium. While the central theme of the interlaboratory study was to provide values to help harmonize lipids, lipid mediators, and precursor measurements across the community, it was also initiated to stimulate a discussion regarding areas in need of improvement.
随着脂质组学领域的不断发展，社群内的自我评估至关重要。在这里，我们使用市售标准参考物质（SRM）1950-冷冻人血浆代谢物进行了脂质组学的实验室间比较研究。该实验室间研究包括 31 个不同的实验室，每个实验室使用不同的脂质组学工作流程。所有实验室共测量了 1527 种独特的脂质，其中 339 种脂质由 5 个或更多参与实验室在总和组成水平上测量，确定了这些脂质的共识定位估计值及其相关不确定性。这些在 SRM 1950 中检测到的评估脂质可作为社区内用于内部和实验室间质量控制和方法验证的基准。这些分析是使用非标准化的、独立于实验室的工作流程进行的。共识位置也与 LIPID MAPS 联盟之前对 SRM 1950 的检查进行了比较。尽管实验室间研究的核心主题是提供有助于协调社区内脂质、脂质介质和前体测量的价值，但它也启动了有关需要改进的领域的讨论。
