NIST 脂质组学工作流程调查问卷：对全社区方法和观点的评估。
NIST lipidomics workflow questionnaire: an assessment of community-wide methodologies and perspectives.
机构信息
Marine Biochemical Sciences Group, National Institute of Standards and Technology, Hollings Marine Laboratory, 331 Fort Johnson Road, Charleston, SC, 29412, USA.
Organic Chemical Measurement Science Group, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD, 20899, USA.
出版信息
Metabolomics. 2018 Mar 20;14(5):53. doi: 10.1007/s11306-018-1340-1.
INTRODUCTION
Efforts to harmonize lipidomic methodologies have been limited within the community. Here, we aimed to capitalize on the recent National Institute of Standards and Technology lipidomics interlaboratory comparison exercise by implementing a questionnaire that assessed current methodologies, quantitation strategies, standard operating procedures (SOPs), and quality control activities employed by the lipidomics community.
OBJECTIVES
Lipidomics is a rapidly developing field with diverse applications. At present, there are no community-vetted methods to assess measurement comparability or data quality. Thus, a major impetus of this questionnaire was to profile current efforts, highlight areas of need, and establish future objectives in an effort to harmonize lipidomics workflows.
METHODS
The 54-question survey inquired about laboratory demographics, lipidomic methodologies and SOPs, analytical platforms, quantitation, reference materials, quality control procedures, and opinions regarding challenges existing within the community.
RESULTS
A total of 125 laboratories participated in the questionnaire. A broad overview of results highlighted a wide methodological diversity within current lipidomic workflows. The impact of this diversity on lipid measurement and quantitation is currently unknown and needs to be explored further. While some laboratories do incorporate SOPs and quality control activities, these concepts have not been fully embraced by the community. The top five perceived challenges within the lipidomics community were a lack of standardization amongst methods/protocols, lack of lipid standards, software/data handling and quantification, and over-reporting/false positives.
CONCLUSION
The questionnaire provided an overview of current lipidomics methodologies and further promoted the need for community-accepted guidelines and protocols. The questionnaire also served as a platform to help determine and prioritize metrological issues to be investigated.
简介
在该领域内，脂质组学方法的协调工作一直受到限制。在此，我们旨在利用最近美国国家标准与技术研究院（NIST）脂质组学实验室间比对工作的机会，实施一项调查问卷，评估脂质组学领域当前使用的方法、定量策略、标准操作规程（SOP）和质量控制活动。
目的
脂质组学是一个快速发展的领域，具有多种应用。目前，还没有经过社区审查的方法来评估测量的可比性或数据质量。因此，这份调查问卷的主要推动力是描述当前的努力，突出需要解决的领域，并确定未来的目标，以协调脂质组学工作流程。
方法
这份包含 54 个问题的调查问卷询问了实验室的基本情况、脂质组学方法和 SOP、分析平台、定量、参考材料、质量控制程序以及对社区内现有挑战的看法。
结果
共有 125 个实验室参与了这项调查。结果的总体概述突出了当前脂质组学工作流程中广泛的方法多样性。这种多样性对脂质测量和定量的影响目前尚不清楚，需要进一步研究。虽然一些实验室确实采用了 SOP 和质量控制活动，但这些概念尚未被社区完全接受。脂质组学社区中前五个被认为的挑战是方法/方案缺乏标准化、缺乏脂质标准品、软件/数据处理和定量、以及过度报告/假阳性。
结论
该调查问卷提供了当前脂质组学方法的概述，并进一步促进了社区接受的指南和协议的需求。该调查问卷还作为一个平台，有助于确定和优先考虑需要调查的计量学问题。
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