Anh Nguyen Ky, Thu Nguyen Quang, Tien Nguyen Tran Nam, Long Nguyen Phuoc, Nguyen Huy Truong
Faculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City 700000, Vietnam.
Department of Pharmacology and PharmacoGenomics Research Center, Inje University College of Medicine, Busan 47392, Republic of Korea.
Molecules. 2024 Dec 16;29(24):5934. doi: 10.3390/molecules29245934.
Targeted metabolomics and lipidomics are increasingly utilized in clinical research, providing quantitative and comprehensive assessments of metabolic profiles that underlie physiological and pathological mechanisms. These approaches enable the identification of critical metabolites and metabolic alterations essential for accurate diagnosis and precision treatment. Mass spectrometry, in combination with various separation techniques, offers a highly sensitive and specific platform for implementing targeted metabolomics and lipidomics in clinical settings. Nevertheless, challenges persist in areas such as sample collection, quantification, quality control, and data interpretation. This review summarizes recent advances in targeted metabolomics and lipidomics, emphasizing their applications in clinical research. Advancements, including microsampling, dynamic multiple reaction monitoring, and integration of ion mobility mass spectrometry, are highlighted. Additionally, the review discusses the critical importance of data standardization and harmonization for successful clinical implementation.
靶向代谢组学和脂质组学在临床研究中的应用日益广泛，可对构成生理和病理机制基础的代谢谱进行定量和全面评估。这些方法能够识别对准确诊断和精准治疗至关重要的关键代谢物及代谢改变。质谱联用各种分离技术，为在临床环境中实施靶向代谢组学和脂质组学提供了一个高度灵敏且特异的平台。然而，在样本采集、定量、质量控制和数据解读等领域仍存在挑战。本综述总结了靶向代谢组学和脂质组学的最新进展，重点阐述了它们在临床研究中的应用。突出了包括微量采样、动态多反应监测以及离子淌度质谱联用等方面的进展。此外，本综述还讨论了数据标准化和协调统一对于成功临床应用的至关重要性。
