使用小RNA测序进行微小RNA表达分析：发现与基于RT-qPCR的验证

MicroRNA Expression Analysis Using Small RNA Sequencing Discovery and RT-qPCR-Based Validation.

作者信息

Van Goethem Alan, Mestdagh Pieter, Van Maerken Tom, Vandesompele Jo

机构信息

Center for Medical Genetics, Ghent University, Ghent, Belgium.

出版信息

Methods Mol Biol. 2017;1654:197-208. doi: 10.1007/978-1-4939-7231-9_13.

DOI:10.1007/978-1-4939-7231-9_13

PMID:28986791

Abstract

miRNAs are small noncoding RNA molecules that function as regulators of gene expression. Deregulated miRNA expression has been reported in various diseases including cancer. Due to their small size and high degree of homology, accurate quantification of miRNA expression is technically challenging. In this chapter, we present two different technologies for miRNA quantification: small RNA sequencing and RT-qPCR.

摘要

微小RNA（miRNA）是一类小的非编码RNA分子，作为基因表达的调节因子发挥作用。在包括癌症在内的各种疾病中，均已报道存在miRNA表达失调的情况。由于其分子尺寸小且同源性高，对miRNA表达进行准确定量在技术上具有挑战性。在本章中，我们介绍两种用于miRNA定量的不同技术：小RNA测序和逆转录定量聚合酶链反应（RT-qPCR）。