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无菌尼龙拭子与 RODAC 平板在重症监护病房环境表面检测多重耐药菌的比较。

Flocked nylon swabs versus RODAC plates for detection of multidrug-resistant organisms on environmental surfaces in intensive care units.

机构信息

Department of Medicine, Rush University Medical Center, Chicago, IL, USA.

Department of Medicine, Rush University Medical Center, Chicago, IL, USA.

出版信息

J Hosp Infect. 2018 Jan;98(1):105-108. doi: 10.1016/j.jhin.2017.09.028. Epub 2017 Oct 4.

DOI:10.1016/j.jhin.2017.09.028
PMID:28987641
Abstract

To compare two culture methods [nylon fiber flocked swabs with broth enrichment versus RODAC ('replicate organism detection and counting') plates] for recovery of multidrug-resistant organisms, 780 environmental surfaces in 63 rooms of patients on contact precautions in four intensive care units at one hospital were examined. Among sites that had at least one positive culture, swab culture with broth enrichment detected the target organisms more frequently than RODAC plates (37.5% vs 26.0%, P = 0.06). There was moderate agreement between the two methods (κ = 0.44) with agreement better for small or flat surfaces compared to large or irregular surfaces.

摘要

为了比较两种培养方法(含肉汤增菌的尼龙纤维植绒拭子与 RODAC 平板(复制体检测和计数))对多药耐药菌的回收效果,对一家医院 4 个重症监护病房中接触预防措施患者的 63 个房间内的 780 个环境表面进行了检测。在至少有一个阳性培养物的部位中,含肉汤增菌的拭子培养法比 RODAC 平板法更频繁地检测到目标菌(37.5%比 26.0%,P=0.06)。两种方法之间存在中度一致性(κ=0.44),对于小而平坦的表面,其一致性优于大而不规则的表面。

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