Department of Restorative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry - University of São Paulo, Bauru, SP, Brazil.
Department of Post Graduation, Faculdade do Centro Oeste Paulista - FACOP, Piratininga, SP Brazil.
Arch Oral Biol. 2017 Dec;84:118-124. doi: 10.1016/j.archoralbio.2017.09.027. Epub 2017 Sep 25.
The aim of this study was to evaluate the effect of Cranberry and Grape seed-enriched extract gels in inhibiting wear and degradation of demineralized organic matrix (DOM).
225 dentin specimens obtained from bovine incisors were randomly allocated into 5 groups (n=45): 10% Grape seed extract gel (GSE), 10% Cranberry extract gel (CE), 0.012% Chlorhexidine gel (CX), 1.23% NaF gel (F), and no active compound gel (P, placebo). Before the treatments, samples were demineralized by immersion in 0.87M citric acid, pH 2.3 (36h). Then, the studied gels were applied once over dentin for 1min. Next, the samples were immersed in artificial saliva containing collagenase obtained from Clostridium histolyticum for 5days. The response variable for dentin wear was depth of dentin loss measured by profilometry and for collagen degradation was hydroxyproline determination. Data were analyzed by ANOVA followed by Tukey's test and Pearson Correlation Test (p<0.05).
Grape seed extract significantly reduced dentin wear compared to the other groups (p<0.05). Cranberry extract and Chlorhexidine did not differ statistically and were able to reduce wear when compared to NaF and placebo treatments. The hydroxyproline analysis showed that there was no significant difference among groups for all treatments (p<0.05). Correlation analysis showed a significant correlation between the amount of degraded DOM evaluated by profilometry and the determination of hydroxyproline.
Cranberry extract was able to reduce the dentin wear and collagen degradation, likely due to the proanthocyanidin content and its action. Therefore, Cranberry could be suggested as an interesting natural-based agent to prevent dentin erosion.
本研究旨在评估蔓越莓和葡萄籽浓缩提取物凝胶对抑制脱矿有机基质（DOM）磨损和降解的作用。
从牛门牙获得的 225 个牙本质标本被随机分配到 5 个组（n=45）：10%葡萄籽提取物凝胶（GSE）、10%蔓越莓提取物凝胶（CE）、0.012%洗必泰凝胶（CX）、1.23%氟化钠凝胶（F）和无活性化合物凝胶（P，安慰剂）。在治疗前，样本通过浸入 0.87M 柠檬酸（pH 2.3）中进行脱矿处理（36 小时）。然后，将研究用凝胶应用于牙本质表面 1 分钟。接下来，将样本浸入含有来自溶组织梭菌的胶原酶的人工唾液中 5 天。牙本质磨损的反应变量通过轮廓测量法测量牙本质损失深度，胶原蛋白降解的反应变量通过羟脯氨酸测定。数据分析采用方差分析，随后进行 Tukey 检验和 Pearson 相关检验（p<0.05）。
与其他组相比，葡萄籽提取物显著减少牙本质磨损（p<0.05）。蔓越莓提取物和洗必泰在统计学上没有差异，与氟化钠和安慰剂治疗相比，能够减少磨损。羟脯氨酸分析表明，所有处理组之间均无显著差异（p<0.05）。相关分析显示，通过轮廓测量法评估的降解 DOM 量与羟脯氨酸测定之间存在显著相关性。
蔓越莓提取物能够减少牙本质磨损和胶原蛋白降解，可能是由于原花青素含量及其作用。因此，蔓越莓可以作为一种有前途的天然抗牙本质侵蚀剂。
