Department of Restorative, Preventive and Pediatric Dentistry, University of Bern, Bern, Switzerland.
PLoS One. 2023 May 18;18(5):e0285931. doi: 10.1371/journal.pone.0285931. eCollection 2023.
To verify the protective effect of plant extracts associated with fluoride against dental erosion of dentine, in the presence and absence of a salivary pellicle.
Dentine specimens (n = 270) were randomly distributed into 9 experimental groups (n = 30/group): GT (green tea extract); BE (blueberry extract); GSE (grape seed extract); NaF (sodium fluoride); GT+NaF (green tea extract and NaF); BE+NaF (blueberry extract and NaF); GSE+NaF (grape seed extract and NaF); negative control (deionized water); and a positive control (commercialized mouthrinse containing stannous and fluoride). Each group was further divided into two subgroups (n = 15), according to the presence (P) or absence (NP) of salivary pellicle. The specimens were submitted to 10 cycles: 30 min incubation in human saliva (P) or only in humid chamber (NP), 2 min immersion in experimental solutions, 60 min of incubation in saliva (P) or not (NP), and 1 min erosive challenge. Dentine surface loss (dSL-10 and dSL-total), amount of degraded collagen (dColl) and total calcium release (CaR) were evaluated. Data were analyzed with Kruskal-Wallis, Dunn's and Mann-Whitney U tests (p>0.05).
Overall, the negative control presented the highest values of dSL, dColl and CaR, and the plant extracts showed different degrees of dentine protection. For the subgroup NP, GSE showed the best protection of the extracts, and the presence of fluoride generally further improved the protection for all extracts. For the subgroup P, only BE provided protection, while the presence of fluoride had no impact on dSL and dColl, but lowered CaR. The protection of the positive control was more evident on CaR than on dColl.
We can conclude that the plant extracts showed a protective effect against dentine erosion, regardless of the presence of salivary pellicle, and that the fluoride seems to improve their protection.
验证与氟化物相关的植物提取物对牙本质脱矿的保护作用，同时存在和不存在唾液膜的情况下。
牙本质标本（n=270）被随机分配到 9 个实验组（n=30/组）：GT（绿茶提取物）；BE（蓝莓提取物）；GSE（葡萄籽提取物）；NaF（氟化钠）；GT+NaF（绿茶提取物和 NaF）；BE+NaF（蓝莓提取物和 NaF）；GSE+NaF（葡萄籽提取物和 NaF）；阴性对照（去离子水）；阳性对照（含有锡和氟化物的商业化漱口液）。根据是否存在唾液膜（P）或不存在唾液膜（NP），每组进一步分为两个亚组（n=15）。标本进行 10 个循环：在人唾液中孵育 30 分钟（P）或仅在湿室中孵育（NP），2 分钟浸入实验溶液中，60 分钟在唾液中孵育（P）或不在（NP），1 分钟侵蚀挑战。评估牙本质表面损失（dSL-10 和 dSL-total）、降解胶原蛋白量（dColl）和总钙释放量（CaR）。使用 Kruskal-Wallis、Dunn's 和 Mann-Whitney U 检验（p>0.05）分析数据。
总体而言，阴性对照呈现出最大的 dSL、dColl 和 CaR 值，而植物提取物显示出不同程度的牙本质保护。对于 NP 亚组，GSE 显示出最好的提取物保护作用，而氟化物的存在通常进一步提高了所有提取物的保护作用。对于 P 亚组，只有 BE 提供了保护，而氟化物的存在对 dSL 和 dColl 没有影响，但降低了 CaR。阳性对照对 CaR 的保护作用比对 dColl 的保护作用更明显。
我们可以得出结论，无论是否存在唾液膜，植物提取物对牙本质脱矿都有保护作用，而氟化物似乎可以提高其保护作用。
