Hox-5.1在小鼠2号染色体上定义了一个含同源异型框的基因位点。

Featherstone M S, Baron A, Gaunt S J, Mattei M G, Duboule D

Laboratoire de Génétique Moléculaire des Eucaryotes, Unité 184 de l'Institut National de la Santé et de la Recherche Médicale, Faculté de Médicine, Strasbourg, France.

Proc Natl Acad Sci U S A. 1988 Jul;85(13):4760-4. doi: 10.1073/pnas.85.13.4760.

We have isolated a murine homeobox-containing gene, Hox-5.1, by virtue of its relatedness to the Hox-1.4 gene. In situ hybridization to metaphase spreads mapped Hox-5.1 to band D of mouse chromosome 2. Sequence comparisons indicate that Hox-5.1 is the murine homolog of the human C13 homeobox-containing gene. Hox-5.1 also bears significant similarity to the Xenopus Xhox-1A homeobox-containing gene and the Drosophila deformed homeotic gene at N-terminal and homeobox regions. Hox-5.1 transcripts were detected in mouse embryos, in adult mouse testis, kidney, heart, and intestine, and in mouse embryonal carcinoma cells treated with retinoic acid. In situ hybridization to sections from whole mouse embryos revealed Hox-5.1 expression in spinal cord and prevertebrae.

我们通过与Hox-1.4基因的相关性，分离出了一个含小鼠同源异型框的基因Hox-5.1。对中期染色体铺片进行原位杂交，将Hox-5.1定位于小鼠2号染色体的D带。序列比较表明，Hox-5.1是人类含C13同源异型框基因的小鼠同源物。Hox-5.1在N端和同源异型框区域也与非洲爪蟾的Xhox-1A含同源异型框基因以及果蝇的变形同源异型基因具有显著相似性。在小鼠胚胎、成年小鼠的睾丸、肾脏、心脏和肠道以及用视黄酸处理的小鼠胚胎癌细胞中检测到了Hox-5.1转录本。对整个小鼠胚胎切片进行原位杂交显示，Hox-5.1在脊髓和椎前体中表达。