海带多糖抗肠道病毒 71 型的抗病毒活性。
Antiviral activity of a polysaccharide from Laminaria japonica against enterovirus 71.
机构信息
Key Laboratory of Rare and Uncommon Diseases, Department of Microbiology, Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan, China.
Key Laboratory of Rare and Uncommon Diseases, Department of Microbiology, Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan, China; Clinical Laboratory, Liaocheng People's Hospital of Taishan Medical University, Liaocheng, Shandong, China.
出版信息
Biomed Pharmacother. 2017 Dec;96:256-262. doi: 10.1016/j.biopha.2017.09.117. Epub 2017 Oct 6.
This in vitro study investigated the antiviral activity of an acidic polysaccharide from Laminaria japonica against enterovirus 71 (EV71) as well as its mechanism of action. The LJ04 polysaccharide was purified from Laminaria japonica by affinity chromatography. To investigate its antiviral activity, an MTT assay, q-PCR, immunofluorescent staining and western-blot analysis were performed. To define its mechanism of action, ELISA, q-PCR and flow cytometry were conducted. LJ04 had a low EC, high CC and high SI. LJ04 inhibited not only JN200804, but also JN200803 in RD cells, and viral proliferation was strongly inhibited, whereas LJ04 suppressed viral-induced apoptosis as detected by flow cytometry. In conclusion, LJ04 was found to have robust antiviral activity by inhibiting apoptosis and inducing IFN-β expression. Our findings indicate that LJ04 is a good candidate for the treatment of EV71.
本体外研究调查了来自海带的酸性多糖对肠道病毒 71（EV71）的抗病毒活性及其作用机制。LJ04 多糖通过亲和层析从海带中纯化得到。为了研究其抗病毒活性，进行了 MTT 测定、q-PCR、免疫荧光染色和 Western-blot 分析。为了确定其作用机制，进行了 ELISA、q-PCR 和流式细胞术分析。LJ04 具有低 EC、高 CC 和高 SI。LJ04 不仅抑制了 RD 细胞中的 JN200804，也抑制了 JN200803，并且强烈抑制了病毒增殖，而流式细胞术检测表明 LJ04 抑制了病毒诱导的凋亡。总之，LJ04 通过抑制凋亡和诱导 IFN-β 表达被发现具有强大的抗病毒活性。我们的研究结果表明，LJ04 是治疗 EV71 的良好候选药物。
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