枸杞多糖体外抗肠道病毒 71 的抗病毒活性。
Antiviral activity of GuiQi polysaccharides against enterovirus 71 in vitro.
机构信息
School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, 730050, China,
出版信息
Virol Sin. 2013 Dec;28(6):352-9. doi: 10.1007/s12250-013-3376-8. Epub 2013 Nov 18.
In this study, we have investigated the antiviral activity of GuiQi polysaccharides (GQP) upon enterovirus 71 (EV71) in vitro. An assay using methyl thiazolyl tetrazolium (MTT), and analyses of cytopathic effects (CPE) were used to examine the antiviral activity of GQP upon Vero cells infected with EV71. The results revealed that GQP at concentrations below 31.2 μg/mL exhibited significant antiviral effects upon EV71 when applied under three different experimental protocols. GQP was most strongly active in preventing the adsorption of EV71 to target cells and in this respect it was significantly more effective than ribavirin. In addition, it was clear that GQP could inhibit viral replication when added to cells 2 h after infection, but if added at the point of infection its effect was weak. GQP is considered to be less toxic than ribavirin, and may warrant further evaluation as a possible agent in the treatment of hand, foot and mouth disease (HFMD).
在这项研究中，我们研究了枸杞多糖（GQP）对肠道病毒 71（EV71）的体外抗病毒活性。采用噻唑蓝（MTT）法和细胞病变效应（CPE）分析，检测 GQP 对感染 EV71 的 Vero 细胞的抗病毒活性。结果表明，GQP 在浓度低于 31.2μg/ml 时，在三种不同的实验方案下对 EV71 具有显著的抗病毒作用。GQP 在预防 EV71 吸附到靶细胞方面最为有效，在这方面比利巴韦林更有效。此外，当在感染后 2 小时加入细胞时，GQP 显然可以抑制病毒复制，但如果在感染时加入，其效果较弱。GQP 的毒性比利巴韦林低，可能有必要进一步评估其作为治疗手足口病（HFMD）的一种可能药物。
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