Key Laboratory of Environmental Exposure and Health of Guangdong Province, School of Environment, Jinan University, Guangzhou 510632, China.
Key Laboratory of Environmental Exposure and Health of Guangdong Province, School of Environment, Jinan University, Guangzhou 510632, China; Joint Genome Institute, Lawrence Berkeley National Laboratory, Walnut Creek 94598, CA, USA.
Sci Total Environ. 2018 Feb 15;615:508-516. doi: 10.1016/j.scitotenv.2017.10.004. Epub 2017 Oct 5.
Triclosan is a common antibacterial agent widely applied in various household and personal care products. The molecule, cell, organ and organism-level understanding of its toxicity pose to some target organisms has been investigated, whereas, the alteration of a single metabolic reaction, gene or protein cannot reflect the impact of triclosan on metabolic network. To clarify the interaction between triclosan stress and metabolism at network and system levels, phospholipid synthesis, and cellular proteome and metabolism of Bacillus thuringiensis under 1μM of triclosan stress were investigated through omics approaches. The results showed that C14:0, C16:1ω7, C16:0 and C18:2ω6 were significantly up-produced, and 19 proteins were differentially expressed. Whereas, energy supply, protein repair and the synthesis of DNA, RNA and protein were down-regulated. PyrH and Eno could be biomarkers to reflect triclosan stress. At network level, the target proteins ACOX1, AHR, CAR, CYP1A, CYP1B1, DNMT1, ENO, HSP60, HSP70, SLC5A5, TPO and UGT expressed in different species shared high sequence homology with the same function proteins found in Homo sapiens not only validated their role as biomarkers but also implied the potential impact of triclosan on the metabolic pathways and network of humans. These findings provided novel insights into the metabolic influence of triclosan at network levels, and developed an omics approach to evaluate the safety of target compound.
三氯生是一种常见的抗菌剂，广泛应用于各种家庭和个人护理产品。人们已经研究了其对某些目标生物体的毒性的分子、细胞、器官和生物体水平的理解，但是单个代谢反应、基因或蛋白质的改变并不能反映三氯生对代谢网络的影响。为了阐明三氯生胁迫与代谢网络和系统水平的相互作用，采用组学方法研究了 1μM 三氯生胁迫下苏云金芽孢杆菌的磷脂合成、细胞蛋白质组和代谢。结果表明，C14:0、C16:1ω7、C16:0 和 C18:2ω6 显著上调，有 19 种蛋白质差异表达。而能量供应、蛋白质修复以及 DNA、RNA 和蛋白质的合成均下调。PyrH 和 Eno 可以作为反映三氯生胁迫的生物标志物。在网络水平上，在不同物种中表达的靶蛋白 ACOX1、AHR、CAR、CYP1A、CYP1B1、DNMT1、ENO、HSP60、HSP70、SLC5A5、TPO 和 UGT 与在智人中发现的具有相同功能的蛋白质具有高度的序列同源性，不仅验证了它们作为生物标志物的作用，还暗示了三氯生对人类代谢途径和网络的潜在影响。这些发现为三氯生在网络水平上的代谢影响提供了新的见解，并开发了一种组学方法来评估目标化合物的安全性。
