Institute of Orthopedic Diseases and Department of Bone and Joint Surgery, The First Affiliated Hospital, Jinan University, Guangzhou 510630, China.
Guangdong Provincial Key Laboratory of Petrochemical Pollution Processes and Control, School of Environmental Science and Engineering, Guangdong University of Petrochemical Technology, Maoming 525000, China.
Environ Int. 2020 Dec;145:106167. doi: 10.1016/j.envint.2020.106167. Epub 2020 Oct 6.
The biological toxicity of deltamethrin at molecular level has been investigated, whereas, the proteome responsive mechanisms of cells under deltamethrin stress at the phylogenetic level are not clear. The proteome expression, transformation-related pathway and regulatory network of Bacillus thuringiensis during the process of deltamethrin transformation were explored using proteomics and metabolomics approaches in the present study. The results showed that deltamethrin was effectively removed by B. thuringiensis within 48 h. The stress responses of B. thuringiensis were activated to resist deltamethrin stress, with significant differential expression of proteins that were primarily involved in the synthesis of DNA and shock proteins, endospore formation, carbon metabolism. The expression patterns of ribosomal proteins confirmed that the transcription and translation of DNA, and biosynthesis of heat shock proteins were inhibited as deltamethrin transformation. The synthesis of oxaloacetate and acetyl-CoA were also hindered, resulting in downregulated expression of carbohydrate metabolism, TCA cycle and energy metabolism. Meanwhile, endospore formation and germination were promoted to resist oxidative stress induced by deltamethrin. These findings imparted novel insight to elucidate underlying stress response mechanisms of the organism under target contaminants stress, and the interaction between deltamethrin transformation and cellular metabolism at the pathway and network levels.
本研究采用蛋白质组学和代谢组学方法，探讨了苏云金芽孢杆菌在降解过程中的蛋白质组表达、转化相关途径和调控网络，探究了拟除虫菊酯在分子水平上的生物毒性，而在系统发育水平上，细胞在拟除虫菊酯胁迫下的蛋白质组响应机制尚不清楚。结果表明，苏云金芽孢杆菌在 48 小时内有效地去除了拟除虫菊酯。苏云金芽孢杆菌的应激反应被激活以抵抗拟除虫菊酯胁迫，主要涉及 DNA 和休克蛋白合成、芽孢形成、碳代谢的蛋白质发生显著差异表达。核糖体蛋白的表达模式证实，拟除虫菊酯转化抑制了 DNA 的转录和翻译以及热休克蛋白的生物合成。草酰乙酸和乙酰辅酶 A 的合成也受到阻碍，导致碳水化合物代谢、三羧酸循环和能量代谢的表达下调。同时，促进芽孢形成和发芽以抵抗拟除虫菊酯诱导的氧化应激。这些发现为阐明目标污染物胁迫下生物的应激响应机制以及拟除虫菊酯转化与细胞代谢在途径和网络水平上的相互作用提供了新的见解。
