Department of Ophthalmology, Tokyo Dental College, Ichikawa General Hospital, Chiba, Japan; Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan.
Department of Ophthalmology, Tokyo Dental College, Ichikawa General Hospital, Chiba, Japan; Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan.
Am J Ophthalmol. 2019 Feb;198:174-180. doi: 10.1016/j.ajo.2018.10.008. Epub 2018 Oct 12.
We sought to investigate the association between the severity of bullous keratopathy and proinflammatory cytokine levels in the aqueous humor (AqH).
Cross-sectional study.
This study included a total of 95 eyes: 62 with bullous keratopathy and 33 that underwent cataract surgery. Central corneal thickness (CCT) and central corneal volume within 4 and 6 mm (CCV and CCV, respectively) were determined using anterior segment optical coherence tomography. A total of 95 AqH samples were collected at the beginning of surgery. The levels of cytokines (interleukins [ILs]-1α, -1β, -4, -6, -8, -10, -12p70, -13, -17A, interferon [IFN]-α, IFN-γ, monocyte chemotactic protein [MCP]-1, E-selectin, P-selectin, and soluble intercellular adhesion molecule-1 [sICAM-1]) in the AqH were measured using multiplex beads immunoassay. We evaluated the correlation among AqH cytokine levels, CCT, CCV, and CCV in eyes with bullous keratopathy.
The levels of protein, ILs-4, -6, -8, -10, -12p70, and -17A, MCP-1, IFN-γ, E-selectin, P-selectin, and sICAM-1 were significantly higher in eyes with bullous keratopathy compared with those of the normal control subjects (all P < .0025). CCT was significantly correlated with the levels of IL-13 (r = 0.551, P = .0009) and sICAM-1 (r = 0.448, P = .0005). CCV was significantly correlated with the levels of IL-13 (r = 0.514, P = .0022) and sICAM-1 (r = 0.404, P = .0019). CCV was significantly correlated with the level of sICAM-1 (r = 0.459, P = .0003).
The severity of corneal edema in eyes with bullous keratopathy was associated with the levels of specific cytokines in the AqH.
我们旨在研究前房水中促炎细胞因子水平与大疱性角膜病变严重程度之间的相关性。
横断面研究。
本研究共纳入 95 只眼：62 只患有大疱性角膜病变，33 只接受白内障手术。使用眼前节光学相干断层扫描测量中央角膜厚度（CCT）和 4 至 6 毫米中央角膜体积（CCV 和 CCV）。在手术开始时共采集 95 个前房水样液（AqH）样本。使用多重珠免疫分析测量 AqH 中细胞因子（白细胞介素[ILs]-1α、-1β、-4、-6、-8、-10、-12p70、-13、-17A、干扰素[IFN]-α、IFN-γ、单核细胞趋化蛋白[MCP]-1、E-选择素、P-选择素和可溶性细胞间黏附分子-1[sICAM-1]）的水平。我们评估了大疱性角膜病变眼中 AqH 细胞因子水平、CCT、CCV 和 CCV 之间的相关性。
大疱性角膜病变眼中的蛋白质、ILs-4、-6、-8、-10、-12p70 和 -17A、MCP-1、IFN-γ、E-选择素、P-选择素和 sICAM-1 的水平明显高于正常对照组（均 P<.0025）。CCT 与 IL-13 水平显著相关（r=0.551，P=0.0009）和 sICAM-1（r=0.448，P=0.0005）。CCV 与 IL-13 水平显著相关（r=0.514，P=0.0022）和 sICAM-1（r=0.404，P=0.0019）。CCV 与 sICAM-1 水平显著相关（r=0.459，P=0.0003）。
大疱性角膜病变眼中角膜水肿的严重程度与前房水中特定细胞因子的水平有关。
