Department of Ophthalmology, Tokyo Dental College Ichikawa General Hospital, Chiba, Japan.
Department of Ophthalmology, Tokyo Dental College Ichikawa General Hospital, Chiba, Japan.
Ocul Surf. 2020 Oct;18(4):801-807. doi: 10.1016/j.jtos.2020.06.010. Epub 2020 Jul 28.
To evaluate the correlation between tear fluid and aqueous humor (AqH) cytokine levels in eyes with bullous keratopathy (BK) and with normal endothelium.
This prospective consecutive case-series study included 71 eyes of 71 patients: 31 eyes with BK, 18 eyes with non-BK corneal diseases, and 22 eyes with uncomplicated cataract (healthy controls). Total protein and cytokine (interleukin [IL]-1α, IL-1β, IL-4, IL-6, IL-8, IL-10, MIP-1α, MIP-1β, monocyte chemotactic protein [MCP]-1, E-selectin, P-selectin, soluble intercellular adhesion molecule [sICAM]-1, and IP-10) levels in the tear fluid and AqH were measured using multiplex beads immunoassay. The correlations between tear and AqH cytokine levels were assessed.
The AqH protein level was significantly higher in BK eyes (1.09 ± 0.08 mg/mL) than in non-BK (0.63 ± 0.11, P = 0.0004) and healthy control (0.62 ± 0.06, P = 0.0002) eyes. The tear total protein and IL-4 levels were significantly higher in the BK group compared to healthy controls (P = 0.0374 and 0.0032, respectively). The AqH IL-8 and sICAM-1 levels were significantly higher in the BK group compared to controls (P = 0.0001 and 0.0083, respectively). In BK eyes, the tear IL-4 level was significantly correlated with the MCP-1(r = 0.563, P = 0.001) and total protein (r = 0.589, P = 0.001) AqH levels. The tear IL-8 level was significantly correlated with the MCP-1(r = 0.598, p = 0.001) and IL-4 (r = 0.781, p < 0.0001) AqH levels in BK eyes. However, no significant correlations were found between tear and AqH cytokine levels in non-BK and healthy controls eyes.
The tear cytokine levels are correlated with those of AqH only in BK, but not in non-BK and healthy controls.
评估大疱性角膜病变（BK）和正常内皮眼的泪液和房水（AqH）细胞因子水平之间的相关性。
这项前瞻性连续病例系列研究纳入了 71 名患者的 71 只眼：31 只 BK 眼，18 只非 BK 角膜疾病眼和 22 只单纯白内障（健康对照）眼。使用多重微珠免疫分析测量泪液和 AqH 中的总蛋白和细胞因子（白细胞介素[IL]-1α、IL-1β、IL-4、IL-6、IL-8、IL-10、巨噬细胞炎性蛋白-1α[MIP-1α]、MIP-1β、单核细胞趋化蛋白-1[MCP]-1、E-选择素、P-选择素、可溶性细胞间黏附分子[sICAM]-1和 IP-10）水平。评估了泪液和 AqH 细胞因子水平之间的相关性。
BK 眼的 AqH 蛋白水平（1.09±0.08mg/mL）明显高于非 BK（0.63±0.11，P=0.0004）和健康对照组（0.62±0.06，P=0.0002）。与健康对照组相比，BK 组的泪液总蛋白和 IL-4 水平明显更高（P=0.0374 和 0.0032）。BK 组的 AqH IL-8 和 sICAM-1 水平明显高于对照组（P=0.0001 和 0.0083）。在 BK 眼中，泪液中 IL-4 水平与 MCP-1（r=0.563，P=0.001）和总蛋白（r=0.589，P=0.001）的 AqH 水平呈显著相关。BK 眼中的泪液 IL-8 水平与 MCP-1（r=0.598，p=0.001）和 IL-4（r=0.781，p<0.0001）的 AqH 水平呈显著相关。然而，在非 BK 和健康对照组眼中，未发现泪液和 AqH 细胞因子水平之间存在显著相关性。
在 BK 眼中，泪液细胞因子水平与 AqH 细胞因子水平相关，而非 BK 和健康对照组中则无相关性。
