穿透性角膜移植术前房水细胞因子水平与内皮细胞快速减少有关。
Preoperative Aqueous Cytokine Levels Are Associated With a Rapid Reduction in Endothelial Cells After Penetrating Keratoplasty.
作者信息
Yagi-Yaguchi Yukari, Yamaguchi Takefumi, Higa Kazunari, Suzuki Terumasa, Yazu Hiroyuki, Aketa Naohiko, Satake Yoshiyuki, Tsubota Kazuo, Shimazaki Jun
机构信息
Department of Ophthalmology, Tokyo Dental College, Ichikawa General Hospital, Chiba, Japan; Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan.
Department of Ophthalmology, Tokyo Dental College, Ichikawa General Hospital, Chiba, Japan; Department of Ophthalmology, Keio University School of Medicine, Tokyo, Japan.
出版信息
Am J Ophthalmol. 2017 Sep;181:166-173. doi: 10.1016/j.ajo.2017.07.005. Epub 2017 Jul 19.
PURPOSE
To evaluate the influence of preoperative inflammatory cytokine levels in the aqueous humor (AqH) on the endothelial cell density (ECD) after penetrating keratoplasty (PKP).
DESIGN
Prospective, interventional, consecutive case series.
METHODS
This study includes 70 consecutive patients (mean age 73.7 ± 10.6 years) who underwent PKP (37 eyes) or cataract surgery (controls, 33 eyes). A total of 70 AqH samples were collected at the beginning of each surgery. The levels of cytokines (interleukin [IL]-1α, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-12p70, IL-13, IL-17A, interferon [IFN]-α, IFN-γ, monocyte chemotactic protein [MCP]-1, E-selectin and P-selectin) in AqH were measured by multiplex bead immunoassay. The subjects who underwent PKP were classified into 2 groups: ECD ≥ 1200 cells/mm at 6 months (24 eyes), and ECD < 1200 cells/mm at 6 months (13 eyes).
RESULTS
The ECD at 3 months significantly correlated with the preoperative levels of IL-10 (r = -0.428, P = .02) and IFN-γ (r = -0.412, P = .029). The ECD at 6 months significantly correlated with the preoperative levels of IL-10 (r = -0.399, P = .024), MCP-1 (r = -0.444, P = .011), and IFN-γ (r = -0.474, P = .006). The preoperative levels of IL-6, IL-10, MCP-1, IFN-γ, and P-selectin in AqH were significantly higher in eyes with ECD < 1200 cells/mm compared with those with ECD ≥ 1200 cells/mm at 6 months (P < .05).
CONCLUSIONS
Higher preoperative levels of IL-10, MCP-1, and IFN-γ in the AqH were associated with low ECD after PKP.
目的
评估穿透性角膜移植术（PKP）前房水（AqH）中炎性细胞因子水平对内皮细胞密度（ECD）的影响。
设计
前瞻性、干预性、连续病例系列研究。
方法
本研究纳入70例连续患者（平均年龄73.7±10.6岁），其中37眼接受PKP，33眼作为白内障手术对照组。在每次手术开始时共采集70份AqH样本。采用多重微珠免疫测定法检测AqH中细胞因子（白细胞介素[IL]-1α、IL-1β、IL-4、IL-6、IL-8、IL-10、IL-12p70、IL-13、IL-17A、干扰素[IFN]-α、IFN-γ、单核细胞趋化蛋白[MCP]-1、E-选择素和P-选择素）水平。接受PKP的患者分为两组：术后6个月ECD≥1200个细胞/mm²（24眼），以及术后6个月ECD<1200个细胞/mm²（13眼）。
结果
术后3个月的ECD与术前IL-10水平（r=-0.428，P=0.02）和IFN-γ水平（r=-0.412，P=0.029）显著相关；术后6个月的ECD与术前IL-10水平（r=-0.399，P=0.024）、MCP-1水平（r=-0.444，P=0.011）和IFN-γ水平（r=-0.474，P=0.006）显著相关。与术后6个月ECD≥1200个细胞/mm²的眼相比，ECD<1200个细胞/mm²的眼中AqH的术前IL-6、IL-10、MCP-1、IFN-γ和P-选择素水平显著更高（P<0.05）。
结论
PKP术前AqH中较高的IL-10、MCP-1和IFN-γ水平与术后低ECD相关。
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