Móricz Ágnes M, Krüzselyi Dániel, Alberti Ágnes, Darcsi András, Horváth Györgyi, Csontos Péter, Béni Szabolcs, Ott Péter G
Plant Protection Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Herman O. Str. 15, 1022 Budapest, Hungary.
Plant Protection Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, Herman O. Str. 15, 1022 Budapest, Hungary.
J Chromatogr A. 2017 Nov 17;1524:266-272. doi: 10.1016/j.chroma.2017.09.062. Epub 2017 Sep 28.
The antibacterial profiling of Onopordum acanthium L. leaf extract and subsequent targeted identification of active compounds is demonstrated. Thin-layer chromatography (TLC) and off-line overpressured layer chromatography (OPLC) coupled with direct bioautography were utilized for investigation of the extract against eight bacterial strains including two plant and three human pathogens and a soil, a marine and a probiotic human gut bacteria. Antibacterial fractions obtaining infusion-transfusion OPLC were transferred to HPLC-MS/MS analysis that resulted in the characterization of three active compounds and two of them were identified as, linoleic and linolenic acid. OPLC method was adopted to preparative-scale flash chromatography for the isolation of the third active compound, which was identified after a further semi-preparative HPLC purification as the germacranolide sesquiterpene lactone onopordopicrin. Pure onopordopicrin exhibited antibacterial activity that was specified as minimal inhibitory concentration in the liquid phase as well.
展示了刺苞菜蓟叶提取物的抗菌谱分析以及随后对活性化合物的靶向鉴定。采用薄层色谱（TLC）和离线超压液相色谱（OPLC）结合直接生物自显影技术，研究该提取物对包括两种植物病原菌、三种人类病原菌以及一种土壤细菌、一种海洋细菌和一种人类肠道益生菌在内的八种细菌菌株的抗菌活性。将通过输液-输液OPLC获得的抗菌馏分进行HPLC-MS/MS分析，从而鉴定出三种活性化合物，其中两种被鉴定为亚油酸和亚麻酸。采用OPLC方法进行制备规模的快速色谱分离第三种活性化合物，经过进一步的半制备HPLC纯化后，鉴定其为吉马烷型倍半萜内酯刺苞菜蓟苦素。纯刺苞菜蓟苦素也表现出抗菌活性，其在液相中的最低抑菌浓度也已明确。
